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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 20 - 31 (12page)
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The anaerobic germination 1 (AG1) locus, which confers tolerance to the anaerobic germination of rice seed, has beenpreviously identified and a gel-based InDel DNA marker developed for marker-assisted selection (MAS). However, there is a need formarker development for high-throughput genotyping in order to increase the breeding efficiency. Single nucleotide polymorphisms(SNPs)-based Kompetitive Allele Specific PCR (KASP) markers enable high-accuracy and high-throughput analyses for thegenotyping of AG1 locus. In the present study, we developed one AG1 InDel-specific and four flanking KASP markers, which can beused as the foreground and recombination markers, respectively, for the AG1 locus. Of those, three KASP markers developed werevalidated with Dasan-AG1 (BC3F2) segregating lines; the AG1 InDel-specific KASP marker was 100% consistent with the existing AG1gel-based marker, while the two flanking markers showed 70.3 and 66.7 percent consistency to the AG1 gel-based marker, respectively. In addition, the five KASP markers developed were further used to genotype 78 Korean and 95 foreign rice varieties. The genotypingresults with 172 varieties revealed that most of the Korean varieties possessed the AG1 locus; however, a wide variation was observedin the foreign varieties for AG1 locus. Hence, the KASP markers developed for AG1 locus show promise as useful tools for expeditingthe breeding of varieties tolerant to anaerobic germination stress via high-throughput genotyping.
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참고문헌 (25)
참고문헌 신청
Altman DG / 1990 / Practical statistics for medical research / CRC press
Angaji SA / 2010 / QTLs associated with tolerance of flooding during germination in rice(Oryza sativa) / Euphytica 172:159~168
Baltazar MD / 2014 / QTL mapping for tolerance of anaerobic germination from IR64 and the aus landrace Nanhi using SNP genotyping / Euphytica 197:251~260
Frisch M / 1999 / Comparison of selection strategies for marker-assisted backcrossing of a gene / Crop Sci 39:1295~1301
Ge LF / 2008 / Overexpression of the trehalose-6-phosphate phosphatase gene OsTPP1 confers stress tolerance in rice and results in the activation of stress responsive genes / Planta 228:191~201
Angaji SA / 2010 / QTLs associated with tolerance of flooding during germination in rice(Oryza sativa) / Euphytica 172:159~168
Baltazar MD / 2014 / QTL mapping for tolerance of anaerobic germination from IR64 and the aus landrace Nanhi using SNP genotyping / Euphytica 197:251~260
Frisch M / 1999 / Comparison of selection strategies for marker-assisted backcrossing of a gene / Crop Sci 39:1295~1301
Ge LF / 2008 / Overexpression of the trehalose-6-phosphate phosphatase gene OsTPP1 confers stress tolerance in rice and results in the activation of stress responsive genes / Planta 228:191~201
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