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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 206 - 213 (8page)
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The detection of hepatitis B surface antigen (HBsAg) with an immunoassay using magnetite nanoparticles (NPs) that bind hepatitis B surface antibody (anti-HBs) was investigated. Magnetite NPs with an average size of 32 nm were synthesized and functionalized using tetraethyl orthosilicate, 3-aminopropyltriethoxysilane, glutaraldehyde, and streptavidin. The functionalized NPs were conjugated with biotinylated anti-HBs to capture HBsAg. HBsAgs were detected with a sensitivity, specificity and agreement of 98%, 97%, and 97.5%, respectively, when compared to the rapid test. In addition, the novel method could also enrich HBsAgs, using different tube sizes and can facilitate amplification for the detection of microorganisms.
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참고문헌 (26)
참고문헌 신청
Bozza, C. / 2016 / Hepatitis B and cancer : A practical guide for the oncologist / Critical Reviews in Oncology/Hematology 98:137~146
Can, K. / 2009 / Immobilization of albumin on aminosilane modified superparamagnetic magnetite nanoparticles and its characterization / Colloids Surfaces B : Biointerfaces 71:154~159
Chaiet, L. / 1964 / The properties of streptavidin, a biotin-binding protein produced by streptomycefes / Archives of Biochemistry and Biophysics 106:1~5
Chaleawlert-Umpon, S. / 2012 / Preparation of magnetic polymer microspheres with reactive epoxide functional groups for direct immobilization of antibody / Colloids Surfaces A : Physicochemical Engineering Aspects 414:66~74
Crowther, J. R. / 2009 / The ELISA guidebook / Humana Press
Can, K. / 2009 / Immobilization of albumin on aminosilane modified superparamagnetic magnetite nanoparticles and its characterization / Colloids Surfaces B : Biointerfaces 71:154~159
Chaiet, L. / 1964 / The properties of streptavidin, a biotin-binding protein produced by streptomycefes / Archives of Biochemistry and Biophysics 106:1~5
Chaleawlert-Umpon, S. / 2012 / Preparation of magnetic polymer microspheres with reactive epoxide functional groups for direct immobilization of antibody / Colloids Surfaces A : Physicochemical Engineering Aspects 414:66~74
Crowther, J. R. / 2009 / The ELISA guidebook / Humana Press
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