지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 358 - 368 (11page)
이용수
초록· 키워드
오류제보하기
Anti-coronavirus disease-2019 (COVID-19; anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)) antibodies against nucleoprotein (NP) were purified from pig sera. Following the separation of the antibody fraction using a protein-A column, the final yield of the purified antibodies against SARSCoV- 2 NPs was estimated to be 0.26 ± 0.05 % (absolute amount of 143.4 ± 25.2 ng, n=5) from 1 mL of pig sera. The binding activities of the isolated antibodies were confirmed using immunoassay and immunostaining. Based on the specific binding activity to NPs, a quantitative assay was performed using a surface plasmon resonance (SPR) biosensor. From the doseresponse curve, the binding constant (Kd) was calculated to be 185 pM and the limit of detection was estimated to be 1.02 pM. The SPR biosensor with the isolated antibodies against SARS-CoV-2 NPs was applied for the detection of SARS-CoV-2, MERSCoV, and CoV strain 229E in culture fluid.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Monchatre-Leroy, E. / 2017 / Identification of alpha and beta coronavirus in wildlife species in France: bats, rodents, rabbits, and hedgehogs / Viruses 9:364
Chen, Y. / 2020 / Emerging coronaviruses:Genome structure, replication, and pathogenesis / J. Med. Virol 92:418~423
Zeng, W. / 2020 / Biochemical characterization of SARSCoV-2 nucleocapsid protein / Biochem. Biophys. Res. Commun 527:618~623
Shang, B. / 2005 / Characterization and application of monoclonal antibodies against N protein of SARScoronavirus / Biochem. Biophys. Res. Commun 336:110~117
Lu, R. / 2020 / Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding / The Lancet 395:565~574
Chen, Y. / 2020 / Emerging coronaviruses:Genome structure, replication, and pathogenesis / J. Med. Virol 92:418~423
Zeng, W. / 2020 / Biochemical characterization of SARSCoV-2 nucleocapsid protein / Biochem. Biophys. Res. Commun 527:618~623
Shang, B. / 2005 / Characterization and application of monoclonal antibodies against N protein of SARScoronavirus / Biochem. Biophys. Res. Commun 336:110~117
Lu, R. / 2020 / Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding / The Lancet 395:565~574
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Pig Sera-derived Anti-SARS-CoV-2 Antibodies with Controlled Specificity for Competitive Immunoassay
한국생물공학회 학술대회
2021 .04
-
Competitive Immunoassay of SARS‑CoV‑2 Using Pig Sera‑Derived Anti‑SARS‑CoV‑2 Antibodies
BioChip Journal
2021 .01
-
Surface Plasmon Resonance (SPR) Biosensor for the Detection of SARS-CoV-2 Using Autodisplyaed FV-antibodies on Outer Membrane of E. coli
BioChip Journal
2024 .03
-
Isolation of?Antibodies Against the?Spike Protein of?SARS-CoV from?Pig Serum for?Competitive Immunoassay
BioChip Journal
2021 .12
-
Development of a SARS-CoV-2-specific Biosensor for Antigen Detection Using scFv-Fc Fusion Proteins
한국생물공학회 학술대회
2021 .04
-
Establishment of Potent Neutralizing Antibodies against SARS-CoV-2
한국생물공학회 학술대회
2021 .10
-
Development of Various Diagnostic tool of SARS-COV-2 Infection
한국생물공학회 학술대회
2020 .06
-
Oriented Antibody Immobilization-based Lateral Flow Immunoassay for Sensitive Detection of SARS-CoV-2
한국생물공학회 학술대회
2023 .04
-
Development and Characterization of Phage-Display-Derived Novel Human Monoclonal Antibodies against the Receptor Binding Domain of SARS-CoV-2
한국생물공학회 학술대회
2023 .04
-
Development of Human-mouse Chimeric Antibodies Recognizing the SARS-CoV-2 Spike Protein
한국생물공학회 학술대회
2021 .10
-
One-Step Immunoassay for the Detection of SARS-CoV-2 Nucleocapsid Protein Using Screened Fv-Antibodies
BioChip Journal
2024 .06
-
Phylogenetic Relationship of the Complete SARS-CoV-2 Genome Sequences
한국생물공학회 학술대회
2020 .06
-
Detection of Severe Acute Respiratory Syndrome Coronavirus 2 Using Graphene-based Biosensor
한국생물공학회 학술대회
2021 .04
-
Computational Approach to Discover Potent Neutralizing Antibody against All SARS-CoV-2 Variants
한국생물공학회 학술대회
2022 .04
-
Novel Bispecific Human Antibody Platform Specifically Targeting a Fully Open Spike Conformation Potently Neutralizes Multiple SARS-CoV-2 Variants
한국생물공학회 학술대회
2023 .04
-
Converting Weak Neutralizing Antibody against SARS-CoV-2 into Potent Antiviral Using Fc-binding Nanodiscs
한국생물공학회 학술대회
2023 .10
-
Specific antigen assay to assist diagnosis of SARS-CoV-2 infection
한국생물공학회 학술대회
2020 .10
-
SARS-CoV-2 Recovery from the Wastewater
한국생물공학회 학술대회
2022 .09
-
Antibody-Binding Nanodisc Restores Neutralization Efficacy of Antibodies with Reduced Effectiveness Against Evolving SARS-CoV-2 Variants
한국생물공학회 학술대회
2024 .09
-
On the origin and evolution of SARS-CoV-2
Experimental and Molecular Medicine
2021 .04
이 논문의 저자 정보
최근 본 자료
댓글(0)
0