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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 407 - 416 (10page)
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초록· 키워드
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Background The blood clam Barbatia virescens is an ecologically and economically important species in the southern coast of China. Understanding of the genetic structure of B. virescens populations is vital to breeding strategies and conservation programs. Objective To develop and characterize a set of microsatellites loci primers for B. virescens, and provide helpful information for reasonable utilization and protection of B. virescens natural resources. Methods The microsatellites of B. virescens were detected using a RAD-seq approach based on an Illumina sequencing platform. For the test of microsatellite development, we calculated the number of alleles (Na), observed heterozygosities (Ho), expected heterozygosities (He) and exact tests for deviations from Hardy–Weinberg equilibrium (HWE). Twelve polymorphic loci were used to access the genetic diversity and population structure of four B. virescens populations. Results In this study, 50,729 microsatellites of B. virescens were detected. Twenty-two polymorphic microsatellite loci were developed for B. virescens. The number of alleles per locus ranged from 6 to 15, and expected heterozygosities varied from 0. 567 to 0.911. All the PIC values of the 22 loci were greater than 0.5, indicating that these markers were highly informative for further genetic analysis. Twelve loci were selected to analyze genetic diversity and population structure of four B. virescens populations collected from different geographical regions along the southern coast of China. The results showed moderate to high levels of genetic diversity in the four populations (mean Ar = 7.756–8.133; mean Ho = 0.575–0.639; mean He = 0.754–0.775). Pairwise FST estimates indicated that there was significant divergence among the four populations. Conclusion This study not only provides a large scale of sequence information of microsatellites which are valuable for future genetic mapping, trait association and kinship among B. virescens, but also offers useful information for the sustainable management of natural stocks and the development of breeding industry of B. virescens.
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참고문헌 (49)
참고문헌 신청
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Baus E / 2005 / Gene-flow patterns in Atlantic and Mediterranean populations of the Lusitanian sea star Asterina gibbosa / Mol Ecol 14:3373~3382
Berman M / 2014 / Characterisation of the complete mitochondrial genome and 13 microsatellite loci through next-generation sequencing for the New Caledonian spider-ant Leptomyrmex pallens / Mol Biol Rep 41:1179~1187
Bohonak AJ / 1999 / Dispersal, gene flow, and population structure / Q Rev Biol 74:21~45
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Baus E / 2005 / Gene-flow patterns in Atlantic and Mediterranean populations of the Lusitanian sea star Asterina gibbosa / Mol Ecol 14:3373~3382
Berman M / 2014 / Characterisation of the complete mitochondrial genome and 13 microsatellite loci through next-generation sequencing for the New Caledonian spider-ant Leptomyrmex pallens / Mol Biol Rep 41:1179~1187
Bohonak AJ / 1999 / Dispersal, gene flow, and population structure / Q Rev Biol 74:21~45
Brown JE / 2010 / Population genetic history of the dreissenid mussel invasions : expansion patterns across North America / Biol invasions 12:3687~3710
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