지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 713 - 721 (9page)
이용수
초록· 키워드
오류제보하기
Objectives Neuropathic pain (NP) is one of the main challenges towards NP syndrome treatment. miR-340-5p exhibit different expression levels in NP models. Its effects on NP remained unclear. The objective of this study was to explore the potential regulation mechanisms of miR-340-5p in NP. Methods Rat model of chronic constriction injury (CCI) was established to induce NP in vivo. NP levels were assessed using mechanical withdrawal threshold (MWT). The inflammation response in CCI rats were determined by HE staining and ELISA assay. The target genes of miR-340-5p were verified by luciferase report assays. Results In CCI rats, level of miR-340-5p was down-regulated both in spinal cord tissues and isolated microglial cells. Paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) were decreased in CCI rats, which were restored upon miR-340-5p overexpression. miR-340-5p overexpression also decreased inflammation as well as expression levels of COX- 2, IL-1β, TNF-α and IL-6 in CCI rats. Luciferase report assays revealed Rap1A was a target gene of miR-340-5p in the experimental model. Elevated miR-340-5p decreased Rap1A expression level in vitro and in vivo. Overexpression of Rap1A protein restored expression levels of COX-2, IL-1β, TNF-α and IL-6, reduced the PWT and PWL and increased inflammation response in CCI rats. Conclusion miR-340-5p alleviated CCI-induced NP by targeting Rap1A. miR-340-5p and Rap1A may be the potential treatment targets for NP therapeutics.
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등록된 정보가 없습니다.
참고문헌 (29)
참고문헌 신청
Akyol O / 2018 / Neurotrophin-3 provides neuroprotection via TrkC receptor dependent pErk5 activation in a rat surgical brain injury model / Exp Neurol 307:82~89
Hjalte H. Andersen / 2014 / MicroRNAs as modulators and biomarkers of inflammatory and neuropathic pain conditions / Neurobiology of Disease 71:159~168
Arai M / 2013 / The miRNA and mRNA changes in rat hippocampi after chronic constriction injury / Pain Med (Malden, Mass) 14:720~729
C. L. Bailey / 2009 / Activation of Rap1 Promotes Prostate Cancer Metastasis / Cancer Research 69(12):4962~4968
David P. Bartel / 2009 / MicroRNAs: Target Recognition and Regulatory Functions / Cell 136(2):215~233
Hjalte H. Andersen / 2014 / MicroRNAs as modulators and biomarkers of inflammatory and neuropathic pain conditions / Neurobiology of Disease 71:159~168
Arai M / 2013 / The miRNA and mRNA changes in rat hippocampi after chronic constriction injury / Pain Med (Malden, Mass) 14:720~729
C. L. Bailey / 2009 / Activation of Rap1 Promotes Prostate Cancer Metastasis / Cancer Research 69(12):4962~4968
David P. Bartel / 2009 / MicroRNAs: Target Recognition and Regulatory Functions / Cell 136(2):215~233
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