지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Analysis of HCV genotypes can help identify infection routes and the development of treatment methods. However, in some samples with a low titer of HCV RNA, it is difficult to analyze their genotypes. In our previous study about HCV genotyping, we could not identify 12 cases among the 175 HCV NAT reactive samples due to their low titer. In this study, we adopted three different kinds of virus concentration methods to identify the genotypes of the 12 unidentified cases and compared their efficacy. The three virus concentration methods were automatic nucleic acid extraction, polyethyleneimine-magnetic bead-based extraction, and sucrose cushion ultracentrifugation. After virus concentration using every three methods, we analyzed HCV RNA genotypes using the concentrated sample of the best efficacy. Among the 12 cases, six were identified as 1b, four as mixed types, and two were unidentified. Here we could validate that the sample concentration method is useful to identify the HCV genotypes, especially in samples with low HCV RNA titers. Furthermore, considering the convenience, high efficacy, and time-saving, automatic nucleic acid extraction is considered the most useful concentration method for samples with titer lower than 50 IU/mL. (Korean J Blood Transfus 2021;32:43-48)
목차
등록된 정보가 없습니다.
참고문헌 (10)
참고문헌 신청
신선미 / 2019 / HCV 핵산증폭검사 양성 헌혈 혈액의 바이러스 유전자 정량 및 유전자형 분석 / 대한수혈학회지 30(3):205~211
강재원 / 2019 / 수혈감염성 병원체의 국내 수혈감염 잔존 위험도 산출 / 대한수혈학회지 30(2):156~162
Lee SH / 1999 / Effective concentration method for applying PCR to detect viruses in water / Korean J Microbiol 35:41~46
Sanyal D / 1991 / Comparison of ultracentrifugation and polyethylene glycol precipitation for concentration of hepatitis B virus(HBV)DNA for molecular hybridisation tests and the relationship of HBV-DNA to HBe antigen and anti-HBe status / J Med Microbiol 35:291~293
Satoh K / 2003 / Virus concentration using polyethyleneimine-conjugated magnetic beads for improving the sensitivity of nucleic acid amplification tests / J Virol Methods 114:11~19
강재원 / 2019 / 수혈감염성 병원체의 국내 수혈감염 잔존 위험도 산출 / 대한수혈학회지 30(2):156~162
Lee SH / 1999 / Effective concentration method for applying PCR to detect viruses in water / Korean J Microbiol 35:41~46
Sanyal D / 1991 / Comparison of ultracentrifugation and polyethylene glycol precipitation for concentration of hepatitis B virus(HBV)DNA for molecular hybridisation tests and the relationship of HBV-DNA to HBe antigen and anti-HBe status / J Med Microbiol 35:291~293
Satoh K / 2003 / Virus concentration using polyethyleneimine-conjugated magnetic beads for improving the sensitivity of nucleic acid amplification tests / J Virol Methods 114:11~19
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