지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
We examined the effect of fluoxetine, a selective serotonin reuptake inhibitor antidepressant, on neuronal viability in mouse cortical near-pure neuronal cultures. Addition of fluoxetine to the media for 24 hours induced neuronal death in a concentration-dependent manner. To delineate the mechanisms of fluoxetine-induced neuronal death, we investigated the effects of trolox, cycloheximide (CHX), BDNF, z-VAD- FMK, and various metal-chelators on fluoxetine-induced neuronal death. Neuronal death was assessed by MTT assay. The addition of 20 mM fluoxetine to the media for 24 hours induced 60-70% neuronal death, which was associated with the hallmarks of apoptosis, chromatin condensation and DNA laddering. Fluoxetine-induced death was significantly attenuated by CHX, BDNF, or z-VAD-FMK. Treatment with antioxidants, trolox and ascorbate, also markedly attenuated fluoxetine-induced death. Interestingly, some divalent cation chelators (EGTA, Ca-EDTA, and Zn-EDTA) also markedly attenuated the neurotoxicity. Fluoxetine-induced reactive oxygen species (ROS) generation was measured using the fluorescent dye 2’,7’-dichlorofluorescin diacetate. Trolox and bathocuproine disulfonic acid (BCPS), a cell membrane impermeable copper ion chelator, markedly attenuated the ROS production and neuronal death. However, deferoxamine, an iron chelator, did not affect ROS generation or neurotoxicity. We examined the changes in intracellular copper concentration using a copper-selective fluorescent dye, Phen Green FL, which is quenched by free copper ions. Fluoxetine quenched the fluorescence in neuronal cells, and the quenching effect of fluoxetine was reversed by co-treatment with BCPS, however, not by deferoxamine. These findings demonstrate that fluoxetine could induce apoptotic and oxidative neuronal death associated with an influx of copper ions.
목차
등록된 정보가 없습니다.
참고문헌 (29)
참고문헌 신청
Wong DT / 1995 / Prozac(fluoxetine, Lilly 110140), the first selective serotonin uptake inhibitor and an antidepressant drug : twenty years since its first publication / Life Sci 57:411~441
Pancrazio JJ / 1998 / Inhibition of neuronal Na+ channels by antidepressant drugs / J Pharmacol Exp Ther 284:208~214
Deák F / 2000 / Inhibition of voltage-gated calcium channels by fluoxetine in rat hippocampal pyramidal cells / Neuropharmacology 39:1029~1036
Ohno Y / 2007 / Inhibition of astroglial Kir4.1 channels by selective serotonin reuptake inhibitors / Brain Res 1178:44~51
Nahon E / 2005 / Fluoxetine(Prozac)interaction with the mitochondrial voltage-dependent anion channel and protection against apoptotic cell death / FEBS Lett 579:5105~5110
Pancrazio JJ / 1998 / Inhibition of neuronal Na+ channels by antidepressant drugs / J Pharmacol Exp Ther 284:208~214
Deák F / 2000 / Inhibition of voltage-gated calcium channels by fluoxetine in rat hippocampal pyramidal cells / Neuropharmacology 39:1029~1036
Ohno Y / 2007 / Inhibition of astroglial Kir4.1 channels by selective serotonin reuptake inhibitors / Brain Res 1178:44~51
Nahon E / 2005 / Fluoxetine(Prozac)interaction with the mitochondrial voltage-dependent anion channel and protection against apoptotic cell death / FEBS Lett 579:5105~5110
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
The Effect of Antidepressants on Mesenchymal Stem Cell Differentiation
대한골대사학회지
2018 .01
-
NADPH Oxidase Mediates b-Amyloid Peptide-Induced Neuronal Death in Mouse Cortical Cultures
전남의대학술지
2017 .01
-
Protective effect of the aerial parts of Silybum marianum against amyloid β protein (25-35)-induced neuronal death in cultured neurons
Journal of Biomedical and Translational Research
2016 .12
-
Fluoxetine affects cytosolic cAMP, ATP, Ca2+ responses to forskolin, and survival of human ovarian granulosa tumor COV434 cells
The Korean Journal of Physiology & Pharmacology
2021 .01
-
Emerging Hyperprolactinemic Galactorrhea in Obsessive Compulsive Disorder with a Stable Dose of Fluoxetine
Clinical Psychopharmacology and Neuroscience
2015 .01
-
Atomoxetine and Fluoxetine Activate AMPK-ACC-CPT1 Pathway in Human SH-SY5Y and U-87 MG Cells
PSYCHIATRY INVESTIGATION
2023 .04
-
Neuronal Differentiation in ASD
대한소아청소년정신의학회 학술대회논문집
2019 .11
-
Different Ways to Die: Cell Death Pathways and Their Association With Spinal Cord Injury
Neurospine
2023 .06
-
Effects of NADPH Oxidase Inhibitors and Mitochondria-Targeted Antioxidants on Amyloid β1-42-Induced Neuronal Deaths in Mouse Mixed Cortical Cultures
전남의대학술지
2018 .01
-
Pharmacological Properties of CDBT in Hypoxia-induced Neuronal Cell Injury and Their Underlying Mechanisms
대한한방내과학회지
2019 .01
-
Effectiveness range and persistence of transcranial direct current stimulation against neuronal death by the transient cerebral ischemia
한국실험동물학회 학술발표대회 논문집
2018 .01
-
Cordycepin protects against β–amyloid and ibotenic acid– induced hippocampal CA1 pyramidal neuronal hyperactivity
The Korean Journal of Physiology & Pharmacology
2019 .01
-
Korean Red Ginseng alleviates neuroinflammation and promotes cell survival in the intermittent heat stress-induced rat brain by suppressing oxidative stress via estrogen receptor beta and brain-derived neurotrophic factor upregulation
Journal of Ginseng Research
2020 .07
-
High fat diet accelerates and exacerbates microgliosis and neuronal damage/death in the somatosensory cortex after transient forebrain ischemia in gerbils
한국실험동물학회 학술발표대회 논문집
2020 .07
-
Transient forebrain ischemia induces impairment in cognitive performance prior to extensive neuronal cell death in Mongolian gerbil (Meriones unguiculatus)
Journal of Veterinary Science
2018 .07
-
Epicatechin Prevents Methamphetamine-Induced Neuronal Cell Death via Inhibition of ER Stress
Biomolecules & Therapeutics(구 응용약물학회지)
2019 .01
-
Neuronal death in the CNS autonomic control center comes very early after cardiac arrest and is not significantly attenuated by prompt hypothermic treatment in rats
한국실험동물학회 학술발표대회 논문집
2021 .01
-
알코올 투여에 의한 뇌세포 사멸과 이에 대한 천연자생물질의 보호효과
해부·생물인류학
2020 .09
-
Cyanidin-3-glucoside inhibits amyloid β25-35-induced neuronal cell death in cultured rat hippocampal neurons
The Korean Journal of Physiology & Pharmacology
2018 .11
-
HSPE1 exerts a protective role in oxidative stress-induced hippocampal neuronal cell damage
한국실험동물학회 학술발표대회 논문집
2021 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0