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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 183 - 196 (14page)
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Present study was conducted to elucidate the molecular regulation mechanisms and the critical genes involved in regulating wheat early responses to Cadmium (Cd) stress. Both ICP-AES and fluorescence labeling were used to find that the Cd2? influx into wheat roots was significantly suppressed by pre-treatment with or in the presence of the Ca2? channel blocker LaCl3, Verapamil and N-ethylmaleimide. RNA-seq technology was used to identify differentially expressed genes (DEGs) during 12 h of 100 lM Cd stress. Raw reads (n = 80,309,620 were obtained. 108,549 unigenes were identified and classified into 25 COG categories. 8584 DEGs were detected. Many DEGs were involved in defense and detoxification mechanisms including signaling protein kinases, transcription factors, metal transporters and biosynthesis-related enzymes. A Gene Ontology annotation analysis based on the DEGs indicated the presence of many categories including cellular process, cell part and binding, catalytic activity and transporter activity. The Kyoto encyclopedia of genes and genomes pathway analysis identified 107 terms that were enriched for all of the 1018 DEGs. Quantitative real-time PCR of 27 selected DEGs revealed that the expression patterns were consistent with the transcript abundance changes as identified by Solexa analysis.
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참고문헌 (71)
참고문헌 신청
Akhtera MF / 2014 / Localization and chemical speciation of cadmium in the roots of barley and lettuce / Environ Exp Bot 100:10~19
Berkelaar E / 2000 / The relationship between root morphology and cadmium accumulation in seedlings of two durum wheat cultivars / Can J Bot 78:381~387
Bhati KK / 2015 / Genome-wide identification and expression characterization of ABCC-MRP transporters in hexaploid wheat / Front Plant Sci 6:488
Bhattacharyya D / 2013 / De novo transcriptome analysis using 454 pyrosequencing of the Himalayan Mayapple, Podophyllum hexandrum / BMC Genomics 14:748
Bobilya D / 1992 / Zinc transport into endothelial cells is a facilitated process / J Cell Physiol 151:1~7
Berkelaar E / 2000 / The relationship between root morphology and cadmium accumulation in seedlings of two durum wheat cultivars / Can J Bot 78:381~387
Bhati KK / 2015 / Genome-wide identification and expression characterization of ABCC-MRP transporters in hexaploid wheat / Front Plant Sci 6:488
Bhattacharyya D / 2013 / De novo transcriptome analysis using 454 pyrosequencing of the Himalayan Mayapple, Podophyllum hexandrum / BMC Genomics 14:748
Bobilya D / 1992 / Zinc transport into endothelial cells is a facilitated process / J Cell Physiol 151:1~7
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