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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제11호
- 발행연도
- 2015.1
- 수록면
- 1,856 - 1,862 (7page)
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초록· 키워드
오류제보하기
In order to improve the stability of endoglucanase under thermal and acidic conditions, the endoglucanase gene was fused to the N-terminus of the Saccharomyces cerevisiae pir gene, encoding the cell wall protein PIR. The fusion gene was transformed into Pichia pastoris GS115 for expression. A resulting strain with high expression and high activity was identified by examining resistance to Geneticin 418, Congo red staining, and quantitative analysis of enzyme activity. SDS-PAGE analysis revealed that the endoglucanase was successfully displayed on the yeast cell surface. The displayed endoglucanase (DEG) showed maximum activity towards sodium carboxyl methyl cellulose at approximately 275 IU/g cell dry weight. DEG exhibited greater than 60% residual activity in the pH range 2.5-8.5, higher than free endoglucanase (FEG), which had 40% residual activity at the same pH range. The highest tolerated temperature for DEG was 70ºC, much higher than that of FEG, which was approximately 50ºC. Moreover, DEG showed 91.1% activity at 65ºC for 120 min, while FEG only kept 77.8% residual activity over the same period. The half-life of DEG was 270 min at 65ºC, compared with only 150 min for FEG. DEG could be used repeatedly at least three times. These results suggest that the DEG has broad applications as a yeast whole-cell biocatalyst, due to its novel properties of high catalytic efficiency, acid-thermal stabilities, and reusability.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Bai Y / 2011 / Cloning of endo-β-glucanase I gene and expression in Pichia pastoris / Front. Agric. China 5 : 196 ~ 200
Bai Y. / 2011 / DNA shuffling of Thermoascus aurantiacus endo-β-glucanase I gene and the study of enzymic properties / 석사 / Agricultural University of Hebei
Bhat MK / 2000 / Cellulases and related enzymes in biotechnology / Biotechnol. Adv 18 : 355 ~ 383
Ecker M / 2006 / Pir proteins of Saccharomyces cerevisiae are attached to β-1,3-glucan by a new protein?carbohydrate linkage / J. Biol. Chem 281 : 11523 ~ 11529
Gai SA / 2001 / Yeast surface display for protein engineering and characterization / Curr. Opin. Struct. Biol 17 : 467 ~ 473
Bai Y. / 2011 / DNA shuffling of Thermoascus aurantiacus endo-β-glucanase I gene and the study of enzymic properties / 석사 / Agricultural University of Hebei
Bhat MK / 2000 / Cellulases and related enzymes in biotechnology / Biotechnol. Adv 18 : 355 ~ 383
Ecker M / 2006 / Pir proteins of Saccharomyces cerevisiae are attached to β-1,3-glucan by a new protein?carbohydrate linkage / J. Biol. Chem 281 : 11523 ~ 11529
Gai SA / 2001 / Yeast surface display for protein engineering and characterization / Curr. Opin. Struct. Biol 17 : 467 ~ 473
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