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논문 기본 정보

자료유형
학술저널
저자정보
나한흠 (강원대학교) 김근철 (강원대학교)
저널정보
한국유전학회 Genes & Genomics Genes & Genomics Vol.40 No.12
발행연도
2018.1
수록면
1,301 - 1,308 (8page)

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Background Adipocyte differentiation is completed by changing gene expression. Chromatin is closely related to gene expression. Therefore, its structure might be changed for adipocyte differentiation. Mouse 3T3-L1 preadipocytes have been used as a cell model to study molecular mechanisms of adipogenesis. Objective To examine changes of chromatin modification and expression of histone modifying enzymes during adipocyte differentiation. Methods Microscopic analysis and Oil Red O staining were performed to determine distinct phenotype of adipocyte differentiation. RT-PCR and Western blot analysis were used to examine expression levels of histone modifying enzymes during adipocyte differentiation. Histone modifications were examined by immunostaining analysis. Results Expression levels of P300 and cbp were increased during adipocyte differentiation. However, acetylation of histones was not quantitatively changed postdifferentiation of 3T3-L1 cells compared to that at pre-differentiation. RT-PCR and Western blot analyses showed that expression levels of hdac2 and hdac3 were increased during adipocyte differentiation, suggesting histone acetylation at chromatin level was homeostatically controlled by increased expression of both HATs and HDACs. Tri-methylation level of H3K9 (H3K9me3), but not that of H3K27me3, was significantly decreased during adipocyte differentiation. Decreased expression of setdb1 was consistent with reduced pattern of H3K9me3. Knock-down of setdb1 induced adipocyte differentiation. This suggests that setdb1 is a key chromatin modifier that modulates repressive chromatin. Conclusion These results suggest that there exist extensive mechanisms of chromatin modifications for homeostatic balance of chromatin acetylation and deconstruction of repressive chromatin during adipocyte differentiation.

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참고문헌 (37)

참고문헌 신청
Abdou HS / 2013 / A positive regulatory domain in CCAAT/enhancer binding protein beta(C/EBPBeta)is required for the glucocorticoid-mediated displacement of histone deacetylase 1(HDAC1)from the C/ebpalpha promoter and maximum adipogenesis / Endocrinology 154:1454~1464 google schola Ardehali MB / 2017 / Polycomb repressive complex 2 methylates elongin A to regulate transcription / Mol Cell 68:872.e6~884.e6 google schola Bao X / 2015 / The p53-induced lincRNA-p21 derails somatic cell reprogramming by sustaining H3K9me3 and CpG methylation at pluripotency gene promoters / Cell Res 25:80~92 google schola Beyer S / 2016 / Canonical Wnt signalling regulates nuclear export of Setdb1 during skeletal muscle terminal differentiation / Cell Discov 2:16037 google schola A. D. Booth / 2017 / Inhibition of adipose tissue PPARγ prevents increased adipocyte expansion after lipectomy and exacerbates a glucose-intolerant phenotype / Cell Proliferation 50(2):e12325 Crossref

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