Light-controlled relaxation of the rat penile corpus cavernosum using NOBL-1, a novel nitric oxide releaser

Yuji Hotta; Naoya Ieda; Ayako Fukamoto; Tomoya Kataoka; Yoshihiro Kawade; Yasuhiro Maeda; Hidehiko Nakagawa; Kazunori Kimura

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자료유형: 학술저널

저자정보: Yuji Hotta (Nagoya City University) Naoya Ieda (Nagoya City University) Ayako Fukamoto (Nagoya City University) Tomoya Kataoka (Nagoya City University) Yoshihiro Kawade (Nagoya City University) Yasuhiro Maeda (Nagoya City University) Hidehiko Nakagawa (Nagoya City University) Kazunori Kimura (Nagoya City University)

저널정보: 대한비뇨기과학회 Investigative and Clinical Urology Investigative and Clinical Urology Vol.57 No.3

발행연도: 2016.1

수록면: 215 - 220 (6page)

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Purpose: To investigate whether relaxation of the rat penile corpus cavernosum could be controlled with NOBL-1, a novel, light-controllable nitric oxide (NO) releaser. Materials and Methods: Fifteen-week-old male Wistar-ST rats were used. The penile corpus cavernosum was prepared and used in an isometric tension study. After noradrenaline (10-5 M) achieved precontraction, the penile corpus cavernosum was irradiated by light (470–500 nm) with and without NOBL-1 (10-6 M). In addition, we noted rats' responses to light with vardenafil (10-6 M), a phosphodiesterase-5 (PDE-5) inhibitor. Next, responses to light in the presence of a guanylate cyclase inhibitor, ODQ (1H-[1,2,4] oxadiazolo[4,3-a]quinoxalin-1-one) (10-5 M), were measured. All measurements were performed in pretreated L-NAME (10-4 M) conditions to inhibit endogenous NO production. Results: Corpus cavernosal smooth muscle, precontracted with noradrenaline, was unchanged by light irradiation in the absence of NOBL-1. However, in the presence of NOBL-1, corpus cavernosal smooth muscle, precontracted with noradrenaline, relaxed in response to light irradiation. After blue light irradiation ceased, tension returned. In addition, the light response was obviously enhanced in the presence of a PDE-5 inhibitor. Conclusions: This study showed that rat corpus cavernosal smooth muscle relaxation can be light-controlled using NOBL-1, a novel, light sensitive NO releaser. Though further in vivo studies are needed to investigate possible usefulness, NOBL-1 may be prove to be a useful tool for erectile dysfunction therapy, specifically in the field of penile rehabilitation.

참고문헌 (21)

참고문헌 신청

Burnett AL / 1992 / Nitric oxide: a physiologic mediator of penile erection / Science 257:401~403

Ignarro LJ / 1990 / Nitric oxide and cyclic GMP formation upon electrical field stimulation cause relaxation of corpus cavernosum smooth muscle / Biochem Biophys Res Commun 170:843~850

Hurt KJ / 2002 / Akt-dependent phosphorylation of endothelial nitric-oxide synthase mediates penile erection / Proc Natl Acad Sci U S A 99:4061~4066

Hosogai N / 2003 / Enzyme activities of the nitric oxide-cGMP pathway in corpus cavernosum isolated from middle-aged rats / Eur J Pharmacol 473:65~70

Beavo JA / 1995 / Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms / Physiol Rev 75:725~748

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