지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 198 - 207 (10page)
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초록· 키워드
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Matrix metalloproteinase-2 (MMP-2) is closely related to the proliferation and invasion of various types of cancers. The protease is secreted by malignant tumor cells, thus allowing the enzyme to serve as a biomarker for cancer diagnosis. Methods have been developed to analyze MMP-2 activities; however, their applications to disease diagnosis have not been widely demonstrated yet because of the need for highend analytical equipment and labor-intensive processes. In this study, we developed an MMP-2 activity assay system by integrating an engineered autoinhibited β-lactamase which can be activated by MMP-2 in an optical sensing system consisting of reassembled common electronic components, such as a laser diode, a solar cell, and a multimeter. The autoinhibited β-lactamase was immobilized on a polymeric biosensing channel by a polydopamine coating and self-assembled monolayer methods. In the presence of MMP-2, the immobilized autoinhibited β-lactamase was converted to an active form that hydrolyzed the chromogenic cephalosporin CENTA, thereby changing the substrate color from pale yellow (λmax=340 nm) to highly discernible chrome yellow (λmax=405 nm). By reading the interfered laser-light intensity, we were able to analyze MMP-2 activities precisely both with the samples prepared in a buffer solution and also those in urine. These results suggested that the developed system can be used for the quantitative analysis of enzyme activity related to cancer diagnosis.
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참고문헌 (26)
참고문헌 신청
Aaronson, S.A. / 1991 / Growth factors and cancer / Science 254:1146~1153
Duffy, M.J. / 1992 / The role of proteolytic enzymes in cancer invasion and metastasis / Clin. Exp. Metastasis 10:145~155
Rosenberg, S.A. / 1999 / A New Era for Cancer Immunotherapy Based on the Genes that Encode Cancer Antigens / Immunity 10:281~287
Westermarck, J. / 1999 / Regulation of matrix metalloproteinase expression in tumor invasion / FASEB. J 13:781~792
Roy, R. / 2009 / Matrix metalloproteinases as novel biomarkers and potential therapeutic targets in human cancer / J. Clin. Oncol 27:5287~5297
Duffy, M.J. / 1992 / The role of proteolytic enzymes in cancer invasion and metastasis / Clin. Exp. Metastasis 10:145~155
Rosenberg, S.A. / 1999 / A New Era for Cancer Immunotherapy Based on the Genes that Encode Cancer Antigens / Immunity 10:281~287
Westermarck, J. / 1999 / Regulation of matrix metalloproteinase expression in tumor invasion / FASEB. J 13:781~792
Roy, R. / 2009 / Matrix metalloproteinases as novel biomarkers and potential therapeutic targets in human cancer / J. Clin. Oncol 27:5287~5297
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