지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.3
- 수록면
- 57 - 63 (7page)
- DOI
- 10.7841/ksbbj.2020.35.1.57
이용수
초록· 키워드
오류제보하기
Matrix metalloproteinases (MMPs) are the zincdependent endopeptidases that cause the degradation and remodeling of extracellular matrix. They are associated with various physiological and pathological processes. Among the MMPs, gelatinases, MMP-2 and MMP-9, play a major role in the metastasis and invasion of cancer or tumor cells. In this study, the inhibitory effect of the halophyte Ligustrum Japonicum on MMP-2 and MMP-9 activity in phorbol-12-myristate- 13-acetate (PMA)-stimulated HT-1080 cells was estimated by using enzyme-linked immunosorbent assay (ELISA), gelatin zymography, reverse transcription-polymerase chain reaction (RT-PCR) and Western blot assay. Also, the effect of L. japonicum on HT-1080 cell migration was investigated by using wound-healing assay. The dried L. japonicum was extracted with dichloromethane (CH₂Cl2₂) and methanol (MeOH), and the combined crude extracts were fractionated according to the solvent polarity to obtain the n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH) and water fractions. Of the fractions, 85% aq.MeOH fraction most effectively suppressed the enzymatic activity, cell mobility, and mRNA and protein expression of MMP-2 and MMP-9. Therefore, L. japonicum will be used as a valuable source of potential anti-invasive agents.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (18)
참고문헌 신청
Statistics Korea / 2018 / Cause of death statistics report in 2017 :7~9
Deryugina, E. I. / 2015 / Tumor angiogenesis : MMP-mediated induction of intravasation-and metastasis-sustaining neovasculature / Matrix Biol 44:94~112
Westermarck, J. / 1999 / Regulation of matrix metalloproteinase expression in tumor invasion / FASEB J 13:781~792
Verma, R. P. / 2007 / Matrix metalloproteinases(MMPs) : chemical–biological functions and(Q)SARs / Bioorg. Med. Chem 15:2223~2268
McCawley, L. J. / 2001 / Matrix metalloproteinases : they're not just for matrix anymore! / Curr. Opin. Cell Biol 13:534~540
Deryugina, E. I. / 2015 / Tumor angiogenesis : MMP-mediated induction of intravasation-and metastasis-sustaining neovasculature / Matrix Biol 44:94~112
Westermarck, J. / 1999 / Regulation of matrix metalloproteinase expression in tumor invasion / FASEB J 13:781~792
Verma, R. P. / 2007 / Matrix metalloproteinases(MMPs) : chemical–biological functions and(Q)SARs / Bioorg. Med. Chem 15:2223~2268
McCawley, L. J. / 2001 / Matrix metalloproteinases : they're not just for matrix anymore! / Curr. Opin. Cell Biol 13:534~540
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UCI(KEPA) : I410-ECN-0101-2020-470-000539395