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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 222 - 230 (9page)
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The purpose of this study is to evaluate the effects of dexamethasone on mesenchymal stem cells from human gingiva using next-generation sequencing. The verification and suggestion of possible mechanisms was performed. Human gingiva-derived stem cells were treated with a final concentration of 10-7M dexamethasone at 2 and 24 hours. Extraction of RNA, sequencing of mRNA, gene ontology and pathway analysis were performed. Quantification by real-time polymerase chain reaction was conducted for validation. A fold change of two was applied for this study, and a log2 normalized read count of 5 or greater was applied to minimize false counts. Expression of SMAD3 and ESR1 was decreased in dexamethasone at 24 hours. Increased expression of BMP6 and ACHE was noted in dexamethasone at 24 hours. TGF-β signaling was involved in the target genes chosen for osteoblast differentiation. It was clear that the application of dexamethasone produced reduced expression of SMAD3 and ESR1 and enhanced expression of ACHE and BMP6 of human gingiva-derived mesenchymal stem cells. An RNA sequencing strategy can provide new insights into the mechanism of dexamethasone in stem cells originating from dental areas.
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참고문헌 (31)
참고문헌 신청
Li, T. / 2014 / MicroRNA expression profile of dexamethasone-induced human bone marrow-derived mesenchymal stem cells during osteogenic differentiation / J. Cell. Biochem. 115:1683~1691
Ishida, Y. / 1998 / Glucocorticoid-induced osteoporosis: both in vivo and in vitro concentrations of glucocorticoids higher than physiological levels attenuate osteoblast differentiation / J. Bone Miner. Res. 13:1822~1826
Atmani, H. / 2003 / Proliferation and differentiation of osteoblasts and adipocytes in rat bone marrow stromal cell cultures: effects of dexamethasone and calcitriol / J. Cell. Biochem. 89:364~372
Coelho, M.J. / 2000 / Human bone cell cultures in biocompatibility testing. Part II: effect of ascorbic acid, beta-glycerophosphate and dexamethasone on osteoblastic differentiation / Biomaterials 21:1095~1102
Park, J.B. / 2014 / Effects of the combination of dexamethasone and fibroblast growth factor2 on differentiation of osteoprecursor cells / Mol. Med. Rep. 9:659~662
Ishida, Y. / 1998 / Glucocorticoid-induced osteoporosis: both in vivo and in vitro concentrations of glucocorticoids higher than physiological levels attenuate osteoblast differentiation / J. Bone Miner. Res. 13:1822~1826
Atmani, H. / 2003 / Proliferation and differentiation of osteoblasts and adipocytes in rat bone marrow stromal cell cultures: effects of dexamethasone and calcitriol / J. Cell. Biochem. 89:364~372
Coelho, M.J. / 2000 / Human bone cell cultures in biocompatibility testing. Part II: effect of ascorbic acid, beta-glycerophosphate and dexamethasone on osteoblastic differentiation / Biomaterials 21:1095~1102
Park, J.B. / 2014 / Effects of the combination of dexamethasone and fibroblast growth factor2 on differentiation of osteoprecursor cells / Mol. Med. Rep. 9:659~662
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