A Ratiometric Fluorescent Assay for Fluazinam Based on FRET Between CdTe Quantum Dots and Porphyrin

Yue Wang; DANQUN HUO; Huixiang Wu; Hui Liu; Junjie Li

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자료유형: 학술저널

저자정보: Yue Wang (Chongqing University) DANQUN HUO (Chongqing University) Huixiang Wu (Chongqing University) Hui Liu (Chongqing University) Junjie Li (Chongqing University)

저널정보: 성균관대학교 성균나노과학기술원 NANO NANO Vol.12 No.10

발행연도: 2017.1

수록면: 109 - 120 (12page)

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A fluorescent detection system for fluazinam was reported using fluorescence resonance energy transfer (FRET) method based on CdTe quantum dots (CdTe QDs) and 5, 10, 15, 20-tetrakis(4-methacryloyloxy) phenyl porphyrin (TMaPP). TMaPP and water-soluble CdTe QDs were synthesized successfully and characterized using FT-IR, 1H NMR, XPS and TEM, respectively. FRET mechanism between CdTe QDs and TMaPP was confirmed by detailed studies on their fluorescent spectra. After a co-culture of TMaPP and CdTe QDs, fluorescent intensity of CdTe QDs decreased significantly while that of TMaPP increased concomitantly due to altered FRET. Addition of fluazinam led to impaired energy transfer from CdTe QDs to TMaPP and therefore fluorescence recovery of CdTe QDs with fluorescence quenching of TMaPP. The correlation of fluazinam concentration with the fluorescence intensity ratio FQDs / FTMaPP provided the basis for quantitative analysis, and a broad linear range varying from 0.01 μM to 5 μM with a low detection limit of 2.3 nM was obtained. As-reported sensor system demonstrated excellent reproducibility, selectivity and sensitivity in real sample detection.

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