지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 691 - 700 (10page)
이용수
초록· 키워드
오류제보하기
Reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) enables the possibility of generating patient-specific cells. However, the low efficiency issue associated with iPSCs generation has limited iPSCs usage in research and clinical applications. In this study, we developed a high efficiency system to generate iPSCs by using a polydimethylsiloxane stencil. This device could be applied to the localization and reprogramming of human fibroblasts. Herein, a well-defined culture system based on a stencil, which supported efficient reprogramming of fibroblasts into iPSCs with 2–4 fold increase in efficacy over conventional methods, is presented. Subsequently, we prepared a multiple analysis system, which used a micro-patterned scissile microarray to characterize iPSCs. The results showed that iPSCs could be cultured into micro-patterns in a precisely controlled manner on the scissile poly(ethylene terephthalate) sheet, which was cut into pieces for subsequent analyses, indicating that this method allows multiple analyses to establish iPSC pluripotency in the same sample. Our approach provides a simple, cost-effective, but highly efficient system for the generation and characterization of iPSCs, and will serve as a powerful tool for establishing patient- and disease-specific pluripotent stem cells.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Takahashi K / 2006 / Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors / Cell 126:663~676
Takahashi K / 2007 / Induction of pluripotent stem cells from adult human fibroblasts by defined factors / Cell 131:861~872
Kiskinis E / 2010 / Progress toward the clinical application of patient-specific pluripotent stem cells / J Clin Invest 120:51~59
Amabile G / 2009 / Induced pluripotent stem cells: current progress and potential for regenerative medicine / Trends Mol Med 15:59~68
Petit I / 2012 / Induced pluripotent stem cells from hair follicles as a cellular model for neurodevelopmental disorders / Stem Cell Res 8:134~140
Takahashi K / 2007 / Induction of pluripotent stem cells from adult human fibroblasts by defined factors / Cell 131:861~872
Kiskinis E / 2010 / Progress toward the clinical application of patient-specific pluripotent stem cells / J Clin Invest 120:51~59
Amabile G / 2009 / Induced pluripotent stem cells: current progress and potential for regenerative medicine / Trends Mol Med 15:59~68
Petit I / 2012 / Induced pluripotent stem cells from hair follicles as a cellular model for neurodevelopmental disorders / Stem Cell Res 8:134~140
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Cellular Reprogramming and Its Application in Regenerative Medicine
조직공학과 재생의학
2015 .01
-
Characterization of myocardial cells derived from inducible pluripotent stem cells(iPSCs)
한국생물공학회 학술대회
2018 .04
-
iPSC technology-Powerful hand for disease modeling and therapeutic screen
BMB Reports
2015 .01
-
Characterization and Bioreactor Expansion of iPSCs for the Treatment of Osteoarthritis
한국생물공학회 학술대회
2024 .09
-
Generation and characterization of integration-free induced pluripotent stem cells from patients with autoimmune disease
Experimental and Molecular Medicine
2016 .01
-
Study on Quality Control Markers of Cardiomyocytes Derived from Human Inducible Pluripotent Stem Cells (iPSCs)
한국생물공학회 학술대회
2019 .04
-
Concurrent progress of reprogramming and gene correction to overcome therapeutic limitation of mutant ALK2-iPSC
Experimental and Molecular Medicine
2016 .01
-
Fast colony formation during human iPSC generation using magnetic nanoparticle
한국생물공학회 학술대회
2016 .04
-
Efficient Reprogramming of Human Somatic Cells into Induced Pluripotent Stem Cells by Intracellular Delivery of RNA Nanoparticle
한국생물공학회 학술대회
2020 .10
-
Small-molecule-mediated reprogramming: a silver lining for regenerative medicine
Experimental and Molecular Medicine
2020 .02
-
Enhanced Reprogramming Efficiency of Induced Pluripotent Stem Cells (iPSCs) Using Lin28-30Kc19 Fusion Protein
한국생물공학회 학술대회
2016 .04
-
Advances and challenges in modeling inherited peripheral neuropathies using iPSCs
Experimental and Molecular Medicine
2024 .06
-
Rapid Non-Invasive Cell Type Identification for iPSC Cell Therapy with Artificial Intelligence-based Holotomography Analysis
한국생물공학회 학술대회
2022 .09
-
Separating various pluripotent state of iPSCs via magnetization of nanoclusters magnetophoresis devices
한국고분자학회 학술대회 연구논문 초록집
2017 .04
-
Comparison of different culture conditions for human iPSC and MSC
한국생물공학회 학술대회
2018 .04
-
Efficient exogenous DNA-free reprogramming with suicide gene vectors
Experimental and Molecular Medicine
2019 .01
-
Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation
Experimental and Molecular Medicine
2018 .01
-
Efficient generation of human induced pluripotent stem cells using self-assembled siRNA/miRNA nanoparticles
한국생물공학회 학술대회
2018 .10
-
Development of High-throughput Versatile iPSC-based Organoid Platform Using 3D Bioprinting
한국고분자학회 학술대회 연구논문 초록집
2023 .04
이 논문의 저자 정보
최근 본 자료
댓글(0)
0