Non-contact Coculture Reveals a Comprehensive Response of Chondrocytes Induced by Mesenchymal Stem Cells Through Trophic Secretion

Lei Xu; Yuxi Wu; Yanli Liu; Yan Zhou; Zhaoyang Ye; Wen-Song Tan

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자료유형: 학술저널

저자정보: Lei Xu (East China University of Science and Technology) Yuxi Wu (East China University of Science and Technology) Yanli Liu (East China University of Science and Technology) Yan Zhou (East China University of Science and Technology) Zhaoyang Ye (East China University of Science and Technology) Wen-Song Tan (East China University of Science and Technology)

저널정보: 한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제15권 제1호

발행연도: 2018.1

수록면: 37 - 48 (12page)

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Coculture between mesenchymal stem cells (MSCs) and chondrocytes has significant implications in cartilage regeneration. However, a conclusive understanding remains elusive. Previously, we reported that rabbit bone marrowderived MSCs (rbBMSCs) could downregulate the differentiated phenotype of rabbit articular chondrocytes (rbACs) in a non-contact coculture system for the first time. In the present study, a systemic investigation was performed to understand the biological characteristics of chondrocytes in coculture with MSCs. Firstly, cells (MSCs and chondrocytes) from different origins were cocultured in transwell system. Different chondrocytes, when cocultured with different MSCs respectively, consistently demonstrated stimulated proliferation, transformed morphology and declined glycosaminoglycan secretion of chondrocytes. Next, cell surface molecules and the global gene expression of rbACs were characterized. It was found that cocultured rbACs showed a distinct surface molecule profile and global gene expression compared to both dedifferentiated rbACs and rbBMSCs. In the end, cocultured rbACs were passaged and induced to undergo the chondrogenic redifferentiation. Better growth and chondrogenesis ability were confirmed compared with control cells without coculture. Together, chondrocytes display comprehensive changes in coculture with MSCs and the cocultured rbACs are beneficial for cartilage repair.

참고문헌 (35)

참고문헌 신청

Madeira C / 2015 / Advanced cell therapies for articular cartilage regeneration / Trends Biotechnol 33:35~42

Huey DJ / 2012 / Unlike bone, cartilage regeneration remains elusive / Science 338:917~921

Hellingman CA / 2012 / Can one generate stable hyaline cartilage from adult mesenchymal stem cells? A developmental approach / J Tissue Eng Regen Med 6:e1~e11

Hubka KM / 2014 / Enhancing chondrogenic phenotype for cartilage tissue engineering: monoculture and coculture of articular chondrocytes and mesenchymal stem cells / Tissue Eng Part B Rev 20:641~654

Leijten JC / 2013 / Cell sources for articular cartilage repair strategies: shifting from monocultures to cocultures / Tissue Eng Part B Rev 19:31~40

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