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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.6
- 수록면
- 120 - 125 (6page)
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Two-dimensional cultivation is typically used for cell growth, but the method reduces the characteristics of chondrocytes and stem cells, and limits culture area. Therefore, development of three-dimensional culture method is needed to mimic in vivo environment, improve quality of cells and scale-up efficiently. Improving proliferation and chondrogenesis is available by co-culture of chondrocytes and mesenchymal stem cells (MSCs) that leads to interaction between two kinds of cells. However, the co-culture has problems that permeability of sphere diminishes as aggregate size increased and ratio of two kinds of cells composing each spheres is different. In this work, co-cultivation method using controlled sphere composed of chondrocytes and MSCs was established and enhanced chondrogenesis. Periosteum-derived progenitor cells (PDPCs) that are appropriate for cell therapy source of articular cartilage were used as MSCs. Controlled spheres were formed in the hanging-drop plates and shifted for being induced chondrogenesis in 35-mm non-adhesive culture dishes at a rotation rate of 60 rpm. After inducing chondrogenesis, gene expressions related with chondrogenesis were found to be improved and it was apparent that the utilization of controlled spheres promoted chondrogenesis. As a result, available numbers of cells per unit area were increased and chondrogenic differentiation ability was improved compared to typical two-dimensional culture. This approach shows the potential in cartilage regeneration as it can provide sufficient numbers of chondrocytes.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (14)
참고문헌 신청
[학술지(정기간행물)] - Fernandes, T. G. / 2014 / Stem cell bioprocessing for regenerative medicine / J. Chem. Technol. Biotechnol 89 : 34 ~ 47
[학술지(정기간행물)] - Fernndez Vallone, V. B. / 2013 / Mesenchymal stem cells and their use in therapy: What has been achieved? / Differentiation 85 : 1 ~ 10
[학술지(정기간행물)] - Breslin, S. / 2013 / Three-dimensional cell culture:the missing link in drug discovery / Drug Discov. Today 18 : 240 ~ 249
[학술지(정기간행물)] - Page, H. / 2013 / Three-dimensional tissue cultures: current trends and beyond / Cell Tissue Res 352 : 123 ~ 131
[학술지(정기간행물)] - Bartosh, T. J. / 2010 / Aggregation of human mesenchymal stromal cells (MSCs)into 3D spheroids enhances their antiinflammatory properties / Proc. Natl. Acad. Sci. USA 107 : 13724 ~ 13729
[학술지(정기간행물)] - Fernndez Vallone, V. B. / 2013 / Mesenchymal stem cells and their use in therapy: What has been achieved? / Differentiation 85 : 1 ~ 10
[학술지(정기간행물)] - Breslin, S. / 2013 / Three-dimensional cell culture:the missing link in drug discovery / Drug Discov. Today 18 : 240 ~ 249
[학술지(정기간행물)] - Page, H. / 2013 / Three-dimensional tissue cultures: current trends and beyond / Cell Tissue Res 352 : 123 ~ 131
[학술지(정기간행물)] - Bartosh, T. J. / 2010 / Aggregation of human mesenchymal stromal cells (MSCs)into 3D spheroids enhances their antiinflammatory properties / Proc. Natl. Acad. Sci. USA 107 : 13724 ~ 13729
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UCI(KEPA) : I410-ECN-0101-2017-470-000801999