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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 질병관리본부 Osong Public Health and Research Persptectives Osong Public Health and Research Persptectives Vol.7 No.2
- 발행연도
- 2016.1
- 수록면
- 127 - 130 (4page)
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Objectives: Multidrug-resistant tuberculosis (MDR-TB) is a global problem that many countries are challenged with. Rapid and accurate detection of MDR-TB is critical for appropriate treatment and controlling of TB. The aim of the present study was to evaluate the TaqMan allelic discrimination without minor groove binder (MGB) as a rapid, efficient, and low-cost method for detection of drug resistant strains of Mycobacterium tuberculosis. Methods: A total of 112 M. tuberculosis isolates from cases with diagnosed TB were subjected to drug susceptibility testing (DST), using the proportion method. Resistant isolates were tested for characterization of mutations in the rpoB and KatG genes by TaqMan genotyping. Results: Of 112 M. tuberculosis isolates for which DST was performed, three, one, and two isolates were MDR, rifampin (RIF) resistant, and isoniazid (INH) resistant, respectively. According to the threshold cycle (Ct) and curve pattern of mutants, TaqMan probes detect all of the mutations in the analyzed genes (katG 315, AGC→ACC, rpoB 531, TCG→TTG, and rpoB 531, TCG→TGG). Conclusion: The present study suggests that drug-resistant strains of M. tuberculosis can be detected by pattern’s curve or Ct with TaqMan probes without MGB in real-time polymerase chain reaction (PCR).
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참고문헌 (21)
참고문헌 신청
Nasiri MJ / 2014 / Prevalence of drug-resistant tuberculosis in Iran : systematic review and metaanalysis / Am J Infect Control 42(11):1212~1218
Ade´kambi T / 2003 / rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria / J Clin Microbiol 41(12):5699~5708
Palomino JC / 2009 / Molecular detection, identification, and drug resistance detection in Mycobacterium tuberculosis / FEMS Immunol Med Microbiol 56(2):103~111
Catanzaro A / 2015 / Performance comparison of three rapid tests for the diagnosis of drug-resistant tuberculosis / PLoS One 10(8):e0136861
Marı´n M / 2004 / Rapid direct detection of multiple rifampin and isoniazid resistance mutations in Mycobacterium tuberculosis in respiratory samples by realtime PCR / Antimicrob Agents Chemother 48(11):4293~4300
Ade´kambi T / 2003 / rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria / J Clin Microbiol 41(12):5699~5708
Palomino JC / 2009 / Molecular detection, identification, and drug resistance detection in Mycobacterium tuberculosis / FEMS Immunol Med Microbiol 56(2):103~111
Catanzaro A / 2015 / Performance comparison of three rapid tests for the diagnosis of drug-resistant tuberculosis / PLoS One 10(8):e0136861
Marı´n M / 2004 / Rapid direct detection of multiple rifampin and isoniazid resistance mutations in Mycobacterium tuberculosis in respiratory samples by realtime PCR / Antimicrob Agents Chemother 48(11):4293~4300
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