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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 193 - 197 (5page)
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Anopheles funestus sensu stricto is a majormalaria vector in Africa. Recently, two clades were identifiedin this species based on mitochondrial DNA sequencedata. We investigated differences between the two An. funestusclades based on nuclear genes and internal transcribedspacer 2 (ITS2) in the ribosomal DNA. Sampleswere collected from Malawi and Mozambique and werethen used to sequence five nuclear genes (BU21, 9K1, 6Z3,BU021 and BU92) and the ITS2 region. The DNAsequence data was used to assess the diversity of the twoAn. funestus clades. We identified 12, 10, 24, 10, and 21polymorphisms in the two An. funestus clades in the BU21,9K1, 6Z3, BU021, and BU92 genes, respectively. No differencesor polymorphisms were found in the ITS2 regionbetween the two clades. This report assesses the diversityof the two An. funestus clades based on nuclear genes andthe ITS2 region. Single nucleotide polymorphisms werefound between the two clades of An. funestus. This couldbe explained by the existence of remaining introgressionregions in the genomes of the two An. funestus clades andlimited divergent regions. Further investigation is neededto determine the specific status of the two clades within An.
funestus.
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참고문헌 (24)
참고문헌 신청
Ballinger-Crabtree ME / 1992 / Use of genetic polymorphisms detected by the random-amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) for differentiation and identification of Aedes aegypti subspecies and populations / Am J Trop Med Hyg 47:893~901
Beckingham K / 1982 / The cell nucleus / Academic Press :205~263
Brumfield RT / 2003 / The utility of single nucleotide polymorphisms in inferences of population history / Trends Ecol Evol 18:249~256
Choi KS / 2012 / Population genetic structure of the major malaria vector Anopheles funestus s.s. and allied species in southern Africa / Parasit Vectors 5:283
Choi KS / 2013 / Detection of clade types (clades I and II) within Anopheles funestus sensu stricto by the hydrolysis probe analysis (Taqman assay) / Parasit Vectors 6:173
Beckingham K / 1982 / The cell nucleus / Academic Press :205~263
Brumfield RT / 2003 / The utility of single nucleotide polymorphisms in inferences of population history / Trends Ecol Evol 18:249~256
Choi KS / 2012 / Population genetic structure of the major malaria vector Anopheles funestus s.s. and allied species in southern Africa / Parasit Vectors 5:283
Choi KS / 2013 / Detection of clade types (clades I and II) within Anopheles funestus sensu stricto by the hydrolysis probe analysis (Taqman assay) / Parasit Vectors 6:173
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