Target engagement of ginsenosides in mild cognitive impairment using mass spectrometry-based drug affinity responsive target stability

Zhu Zhu; Ruimei Li; Wei Qin; Hantao Zhang; Yao Cheng; Feiyan Chen; Cuihua Chen; Lin Chen; Yunan Zhao

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자료유형: 학술저널

저자정보: Zhu Zhu (Nanjing University of Chinese Medicine) Ruimei Li (Nanjing University of Chinese Medicine) Wei Qin (Nanjing University of Chinese Medicine) Hantao Zhang (Nanjing University of Chinese Medicine) Yao Cheng (Nanjing University of Chinese Medicine) Feiyan Chen (Nanjing University of Chinese Medicine) Cuihua Chen (Nanjing University of Chinese Medicine) Lin Chen (Nanjing University of Chinese Medicine) Yunan Zhao (Nanjing University of Chinese Medicine)

저널정보: 고려인삼학회 Journal of Ginseng Research Journal of Ginseng Research Vol.46 No.6

발행연도: 2022.11

수록면: 750 - 758 (9page)

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Background: Mild cognitive impairment (MCI) is a transitional condition between normality and dementia. Ginseng is known to have effects on attenuating cognitive deficits in neurogenerative diseases. Ginsenosides are the main bioactive component of ginseng, and their protein targets have not been fully understood. Furthermore, no thorough analysis is reported in ginsenoside-related protein targets in MCI.
Methods: The candidate protein targets of ginsenosides in brain tissues were identified by drug affinity responsive target stability (DARTS) coupled with label-free liquid chromatography-mass spectrometry (LCeMS) analysis. Network pharmacology approach was used to collect the therapeutic targets for MCI. Based on the above-mentioned overlapping targets, we built up a proteineprotein interaction (PPI) network in STRING database and conducted gene ontology (GO) enrichment analysis. Finally, we assessed the effects of ginseng total saponins (GTS) and different ginsenosides on mitochondrial function by measuring the activity of the mitochondrial respiratory chain complex and performing molecular docking.
Results: We screened 2526 MCI-related protein targets by databases and 349 ginsenoside-related protein targets by DARTS. On the basis of these 81 overlapping genes, enrichment analysis showed the mitochondria played an important role in GTS-mediated MCI pharmacological process. Mitochondrial function analysis showed GTS, protopanaxatriol (PPT), and Rd increased the activities of complex I in a dose-dependent manner. Molecular docking also predicted the docking pockets between PPT or Rd and mitochondrial respiratory chain complex I.
Conclusion: This study indicated that ginsenosides might alleviate MCI by targeting respiratory chain complex I and regulating mitochondrial function, supporting ginseng"s therapeutic application in cognitive deficits.

#Drug affinity responsive target stability (DARTS) #Mild cognitive impairment #Mitochondria #Target engagement #Ginsenoside

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