지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제8호
- 발행연도
- 2022.8
- 수록면
- 1,026 - 1,033 (8page)
- DOI
- 10.4014/jmb.2207.07013
이용수
초록· 키워드
오류제보하기
This study presents a novel DNA part characterization technique that increases throughput by combinatorial DNA part assembly, solid plate-based quantitative fluorescence assay for phenotyping, and barcode tagging-based long-read sequencing for genotyping. We confirmed that the fluorescence intensities of colonies on plates were comparable to fluorescence at the single-cell level from a high-end, flow-cytometry device and developed a high-throughput image analysis pipeline. The barcode tagging-based long-read sequencing technique enabled rapid identification of all DNA parts and their combinations with a single sequencing experiment. Using our techniques, forty-four DNA parts (21 promoters and 23 RBSs) were successfully characterized in 72 h without any automated equipment. We anticipate that this high-throughput and easy-to-use part characterization technique will contribute to increasing part diversity and be useful for building genetic circuits and metabolic pathways in synthetic biology.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
High-throughput sequencing analysis of differences in intestinal microflora between ulcerative colitis patients with different glucocorticoid response types
Genes & Genomics
2020 .01
-
Cytogenetic Analysis of Bienertia sinuspersici Akhani as the First Step in Genome Sequencing
Genes & Genomics
2020 .01
-
DNA fluorescence switching system for constructing DNA memory chip
한국진공학회 학술발표회초록집
2021 .02
-
Storing Digital Information in Long-Read DNA
Genomics & informatics
2018 .01
-
DNA-based information storage
한국진공학회 학술발표회초록집
2017 .02
-
MinIONTM: New, Long Read, Portable Nucleic Acid Sequencing Device
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2015 .12
-
Structure and function of Zalpha, a Z conformation-specific nucleic acid binding domain
Biodesign
2015 .12
-
DNA barcoding of Schisandraceae in Korea
식물분류학회지
2016 .09
-
Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing
Genomics & informatics
2018 .01
-
Single-Cell Molecular Barcoding to Decode Multimodal Information Defining Cell States
Molecules and Cells
2023 .02
-
High-Throughput Cultivation Based on Flow Cytometry
한국미생물학회 학술대회논문집
2018 .04
-
Genetically-encoded Biosensor Circuits for High Throughput Enzyme Engineering and Synthetic Biology
한국미생물학회 학술대회논문집
2020 .10
-
Exonuclease III-assisted Rapid Detection of HIV DNA by Using DNA-templated Silver Nanoclusters
대한화학회지
2021 .10
-
[INTERVIEW] THE DNA DETECTIVE DNA 탐정
BBC 사이언스
2022 .06
-
DNA-based data encoding system
한국진공학회 학술발표회초록집
2016 .08
-
DNA-Dependent Protein Kinase Catalytic Subunit (DNA-PKcs): Beyond the DNA Double-Strand Break Repair
Molecules and Cells
2023 .04
-
Effects of p-coumaric acid on microRNA expression profiles in SNU-16 human gastric cancer cells
Genes & Genomics
2020 .01
-
A newly developed capture-based sequencing panel for genomic assay of lung cancer
Genes & Genomics
2020 .01
-
Aspergillus aureoterreus, A. pseudonomiae, A. siamensis, and A. flocculosus-ochraceopetaliformis as Four New Pathogens of Eye Infection in DNA Barcoding Markers
대한의진균학회지
2024 .09
-
DNA Strand Breaks in Mitotic Germ Cells of Caenorhabditis elegans Evaluated by Comet Assay
Molecules and Cells
2016 .03
이 논문의 저자 정보
최근 본 자료
댓글(0)
0