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논문 기본 정보

자료유형
학술저널
저자정보
Kim Dae-Hwan (Department of Bioenergy Science and Technology Chonnam National University Gwangju 61186 Republic o) Choi Hyo-Jin (Department of Bioenergy Science and Technology Chonnam National University Gwangju 61186 Republic o) Lee Yu Rim (Interdisciplinary Program of Agriculture and Life Science Chonnam National University Gwangju 61186) 김수정 (전남대학교) Lee Sangmin (Gwangju Bio/Energy R&D Center, Korea Institute of Energy Research, Gwangju 61003, Republic of Korea) 이원흥 (전남대학교)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제11호
발행연도
2022.11
수록면
1,485 - 1,495 (11page)
DOI
10.4014/jmb.2209.09004

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The development of a yeast strain capable of fermenting mixed sugars efficiently is crucial for producing biofuels and value-added materials from cellulosic biomass. Previously, a mutant Pichia stipitis YN14 strain capable of co-fermenting xylose and cellobiose was developed through evolutionary engineering of the wild-type P. stipitis CBS6054 strain, which was incapable of cofermenting xylose and cellobiose. In this study, through genomic and transcriptomic analyses, we sought to investigate the reasons for the improved sugar metabolic performance of the mutant YN14 strain in comparison with the parental CBS6054 strain. Unfortunately, comparative wholegenome sequencing (WGS) showed no mutation in any of the genes involved in the cellobiose metabolism between the two strains. However, comparative RNA sequencing (RNA-seq) revealed that the YN14 strain had 101.2 times and 5.9 times higher expression levels of HXT2.3 and BGL2 genes involved in cellobiose metabolism, and 6.9 times and 75.9 times lower expression levels of COX17 and SOD2.2 genes involved in respiration, respectively, compared with the CBS6054 strain. This may explain how the YN14 strain enhanced cellobiose metabolic performance and shifted the direction of cellobiose metabolic flux from respiration to fermentation in the presence of cellobiose compared with the CBS6054 strain.

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