Catalpol Inhibits Tregs-to-Th17 Cell Transdifferentiation by Up-Regulating Let-7g-5p to Reduce STAT3 Protein Levels

Yuxi Di; Mingfei Zhang; Yichang Chen; Ruonan Sun; Meiyu Shen; Fengxiang Tian; Pei Yang; Feiya Qian; Lingling Zhou

doi:10.3349/ymj.2022.63.1.56

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자료유형: 학술저널

저자정보: Yuxi Di (Nanjing University of Chinese Medicine) Mingfei Zhang (Nanjing University of Chinese Medicine) Yichang Chen (Nanjing University of Chinese Medicine) Ruonan Sun (Nanjing University of Chinese Medicine) Meiyu Shen (Nanjing University of Chinese Medicine) Fengxiang Tian (Nanjing University of Chinese Medicine) Pei Yang (Nanjing University of Chinese Medicine) Feiya Qian (Nanjing University of Chinese Medicine) Lingling Zhou (Nanjing University of Chinese Medicine)

저널정보: 연세대학교 의과대학 Yonsei Medical Journal Yonsei Medical Journal 제63권 제1호

발행연도: 2022.1

수록면: 56 - 65 (10page)

DOI: 10.3349/ymj.2022.63.1.56

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Purpose: Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease, and Th17 cells are key factors in the pathogenesis of human inflammatory conditions, such as RA. Catalpol (CAT), a component in Rehmanniae Radix (RR), has been found to regulate human immunity. However, the effects of CAT on Th17 cell differentiation and improvement of RA are not clear. Materials and Methods: Collagen-induced arthritis (CIA) mice were constructed to detect the effects of CAT on arthritis and Th17 cells. The effect of CAT on Th17 differentiation was evaluated with let-7g-5p transfection experiments. Flow cytometry was used to detect the proportion of Th17 cells after CAT treatment. Levels of interleukin-17 and RORγt were assessed by qRT-PCR and enzyme-linked immunosorbent assay. The expression of signal transducer and activator of transcription 3 (STAT3) was determined by qRT-PCR and Western blot. Results: We found that the proportion of Th17 cells was negatively associated with let-7g-5p expression in CIA mice. In in vitro experiments, CAT suppressed traditional differentiation of Th17 cells. Simultaneously, CAT significantly decreased Tregs-to-Th17 cells transdifferentiation. Our results demonstrated that CAT inhibited Tregs-to-Th17 cells transdifferentiation by up-regulating let-7g-5p and that the suppressive effect of CAT on traditional differentiation of Th17 cells is not related with let-7-5p. Conclusion: Our data indicate that CAT may be a potential modulator of Tregs-to-Th17 cells transdifferentiation by up-regulating let-7g-5p to reduce the expression of STAT3. These results provide new directions for research into RA treatment.

#Collagen-induced arthritis #CAT #Th17 cell differentiation #microRNA let-7g-5p #STAT3

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