메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류

추천
검색

논문 기본 정보

자료유형
학술저널
저자정보
Hye-Ryung Kim (College of Veterinary Medicine & Animal Disease Intervention Center Kyungpook National Universi) Gyu-Tae Jeon (College of Veterinary Medicine & Animal Disease Intervention Center Kyungpook National Universi) Jong-Min Kim (College of Veterinary Medicine & Animal Disease Intervention Center Kyungpook National Universi) Ji-Su Baek (College of Veterinary Medicine & Animal Disease Intervention Center Kyungpook National Universi) Yeun-Kyung Shin (Foreign Animal Disease Division Animal and Plant Quarantine Agency Gimcheon 39660 Korea) Oh-Kyu Kwon (Foreign Animal Disease Division Animal and Plant Quarantine Agency Gimcheon 39660 Korea) Hae-Eun Kang (Foreign Animal Disease Division Animal and Plant Quarantine Agency Gimcheon 39660 Korea) Ho-Seong Cho (College of Veterinary Medicine Jeonbuk National University Iksan 54596 Korea) Doo-Sung Cheo (Postbio Inc. Guri 11906 Korea) Choi-Kyu Park (College of Veterinary Medicine & Animal Disease Intervention Center Kyungpook National Universi)
저널정보
한국동물위생학회(구 한국가축위생학회) 한국가축위생학회지 한국가축위생학회지 제45권 제4호
발행연도
2022.12
수록면
305 - 316 (12page)
DOI
10.7853/kjvs.2022.45.4.305

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색

초록· 키워드

오류제보하기
Bordetella (B .) bronchiseptica , Mycoplasma (M .) felis , and Chlamydia (C .) felis are considered as main bacterial pathogens of feline upper respiratory tract disease (URTD). In this study, a new triplex quantitative real-time polymerase chain reaction (tqPCR) assay was developed for the rapid and dif-ferential detection of these bacteria in a single reaction. The assay specifically amplified three bacte-rial genes with the detection limit of below 10 copies/reaction. The assay showed high repeatability and reproducibility, with coefficients of intra-assay and inter-assay variation of less than 1%. Based on the diagnostic results of the assay using 94 clinical samples obtained from cats with URTD signs, prevalence of B. bronchiseptica , M. felis , or C. felis was 10.6%, 36.2%, or 6.4%, respectively, indicat-ing that the diagnostic sensitivity was comparable to those of previously reported monoplex qPCR assays. The dual infection rates for B. bronchiseptica and M. felis or M. felis and C. felis was 2.1% or 3.2%, respectively. These results indicated that M. felis has been widely spread, and its co-infection with B. bronchiseptica or M. felis has been frequently occurred in Korean cat population. The devel-oped tqPCR assay will serve as a promising tool for etiological and epidemiological studies of these three bacterial pathogens and the prevalence data obtained in this study will contribute to expanding knowledge about the epidemiology of feline URTD in Korea.

목차

등록된 정보가 없습니다.

참고문헌 (0)

참고문헌 신청

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

최근 본 자료

전체보기

댓글(0)

0