지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.3
- 수록면
- 41 - 48 (8page)
- DOI
- 10.1007/s13206-021-00044-x
이용수
초록· 키워드
오류제보하기
Nucleic acid testing (NAT) is important for the identification and quantification of specific nucleic acid targets, both DNA and RNA, in life sciences and clinical diagnostics. Nucleic acid amplification can be a time-consuming step in NAT using the polymerase chain reaction (PCR) assay. Therefore, this study aimed to develop a simple method to reduce the amplification time while maintaining the PCR system. The three-step process of a general qPCR was reduced to a two-step process. The annealing/extension temperatures were increased to minimize the differences between the denaturation temperature and the annealing/extension temperatures. Subsequently, the time for each of these steps was reduced and, finally, the denaturation temperature was lowered. Taq polymerase was replaced with SD polymerase because it has strand displacement activity and is efficient in amplifying partial dsDNA at lower denaturation temperatures. In the two-step qPCR of genomic DNA using SD polymerase, the final conditions included an initial denaturation at 92 °C for 2 min, and 1 s at each cycling step with a denaturation temperature of 87 °C and an annealing/extension temperature of 72 °C. Amplification of the nucleocapsid ( N ) gene of SARS-CoV-2 RNA virus was evaluated at a template concentration as low as 10 copies. This method, named SF-qPCR (strand displacement-based fast quantitative polymerase chain reaction), can stably detect less than 10 copies of DNA and RNA within 25–40 min. This new protocol allows for sensitive and rapid detection of important DNA and RNA targets in clinical diagnosis.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Sustainable Additive Manufacturing via Protein Denaturation
한국고분자학회 학술대회 연구논문 초록집
2024 .09
-
Sensitive Detection of Nucleic Acid Biomarkers Using Transcription Mediated Isothermal Amplification
한국생물공학회 학술대회
2021 .10
-
Development of Hydrogel Microparticle based RT-qPCR for Advanced Detection of BCR-ABL1 Transcripts
BioChip Journal
2019 .01
-
Amplifying Nucleic Acids at Physiological Temperatures Using Low-Temperature Loop-Mediated Isothermal Amplification
한국생물공학회 학술대회
2023 .04
-
Detection of Target DNA by DNA Polymerase Switch
한국생물공학회 학술대회
2016 .04
-
Recombinase Polymerase Amplification on a Paper Chip for Multiplex Detection of Pathogen DNas
한국생물공학회 학술대회
2017 .04
-
Improved PCR Strategies for Disease Diagnosis
한국생물공학회 학술대회
2023 .04
-
Target-specific Signal Amplification Reaction for Real-time Analysis of RNA
한국생물공학회 학술대회
2020 .06
-
Isothermal signal amplification method for real-time target nucleic acid detection based on DNA three way junction structure
한국생물공학회 학술대회
2019 .10
-
Sensitive One-step Detection of Pathogen-derived RNAs
한국생물공학회 학술대회
2019 .10
-
How Z-DNA/RNA binding proteins shape homeostasis, inflammation, and immunity
BMB Reports
2020 .01
-
Protein Detection Based on The Target-induced DNA Polymerase Activity Modulation
한국생물공학회 학술대회
2018 .04
-
Isothermal, One-pot Fluorescence Detection of Pathogen-derived RNAs Based on Cell-free Synthetic Biology
한국생물공학회 학술대회
2021 .10
-
Ultrafast polymerase chain reaction for next-generation molecular diagnostics
한국생물공학회 학술대회
2020 .10
-
A Paper-Strip Device-Based Extraction and Detection of DNA/RNA from Complex Biological Samples
한국생물공학회 학술대회
2019 .04
-
Nucleic acid-based technology
한국생물공학회 학술대회
2018 .04
-
A Rapid and One-pot Method for MicroRNA Detection Using T7 RNA Polymerase and CRISPR-Cas13a
한국생물공학회 학술대회
2024 .09
-
Self-Priming Hairpin-utilized Isothermal Amplification of the G-rich Sequence for Target Nucleic Acid Detection
한국생물공학회 학술대회
2023 .10
-
Highly Sensitive Detection of MicroRNA Using Isothermal Amplification and Toehold-mediated Strand Displacement
한국생물공학회 학술대회
2021 .10
-
저가의 휴대용 PCR 장치 구현을 위한 온도 제어와 설계
한국정밀공학회 학술발표대회 논문집
2022 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0