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논문 기본 정보

자료유형
학술저널
저자정보
Zhao Xiao-mei (Key Lab of Plant Stress Research College of Life Sciences Shandong Normal University No. 88 Wenhuad) Lian Yu-ji (Laboratory of Plant Molecular Genetics and Crop Gene Editing College of Life Sciences Linyi Univers) Jin Ze-lin (Laboratory of Plant Molecular Genetics and Crop Gene Editing College of Life Sciences Linyi Univers) Zhang Xue-jie (Key Lab of Plant Stress Research College of Life Sciences Shandong Normal University No. 88 Wenhuad) Y Yan (Jinan Vocational College No.12 Shungeng Road Lixia District Jinan 250014 Shandong China) Fan Shou-jin (Key Lab of Plant Stress Research College of Life Sciences Shandong Normal University No. 88 Wenhuad)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제16권 제4호
발행연도
2022.8
수록면
389 - 400 (12page)
DOI
10.1007/s11816-021-00727-9

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Leaf material explants of Pulsatilla tongkangensis Y. N. Lee & T. C. Lee were used to regenerate plants of this endangered species by somatic embryogenesis and organogenesis from meristematic nodules, induced by MS medium supplemented with zeatin (Zn) and indole-3-acetic acid (IAA). Globular structures were induced on the surface of the explants after 2?weeks and after 6?7?weeks of culture, multiple shoots developed from the nodules. Morpho-histological analysis of light green globular, heart-shaped structures resembling somatic embryos revealed, however, that these were organogenic, with strongly vacuolated parenchymatous cells surrounded by a single layer of epithermal cells, and tracheid elements, but no root pole. Milky-white callus also developed around the nodules after 4?6?weeks. Morpho-histological analysis of the globular, heart-, and torpedo-shaped stages of regenerants present in this callus confirmed the development of somatic embryos in the milky-white structures, characterized by deeply staining, small cells with rich cytoplasm, very little vascular tissue in the developing embryos, and no vascular connection with the surrounding callus. The highest rooting frequency (93.33%) was achieved on MS medium containing 1.5?mg/l NAA. Plantlets were acclimatized and successfully transferred to pots. Our results provide a plant regeneration system with potential for germplasm conservation of endangered plants and the rapid propagation and molecular breeding of P. tongkangensis.

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