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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2023.3
- 수록면
- 35 - 45 (11page)
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초록· 키워드
오류제보하기
The advanced clinical stage of breast cancer causes a lower survival rate even though the survival rate in the early stages is relatively high. Thus, the development of a rapid and easy-to-access diagnostic method is needed. In this study, a single chain Fv (scFv)-type antibody fragment that binds to HER2, a transmembrane protein overexpressed on breast cancer cells, was generated. The scFv was converted to a Quenchbody by site-specifically labeling the scFv using fluorescent dye through a maleimide-thiol reaction. The Quenchbody could detect a picomolar order of HER2 in a few minutes, and its signal-to-background ratio of fluorescence intensity was higher than a fluorescence scFv generated through a conventional fluorescent dye-conjugating method. The Quenchbody can be used as a probe for detecting and imaging of breast cancer cells for point-of-care diagnosis of breast cancer.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
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