지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.3
- 수록면
- 342 - 350 (9page)
- DOI
- 10.14348/molcells.2018.2320
이용수
초록· 키워드
오류제보하기
Flowering time is determined by florigens. These genes include, Heading date 3a (Hd3a) and Rice FT 1 (RFT1) in rice, which are specifically expressed in the vascular tissues of leaves at the floral transition stage. To study the cis-regulatory elements present in the promoter region of Hd3a, we generated transgenic plants carrying the 1.75-kb promoter fragment of Hd3a that was fused to the β-glucuronidase (GUS) reporter gene. Plants expressing this construct conferred a vascular cell-specific expression pat-tern for the reporter gene. However, GUS was expressed in leaves at all developmental stages, including the early seedling stage when Hd3a was not detected. Furthermore, the reporter was expressed in roots at all stages. This suggests that the 1.75-kb region lackings cis-elements that regulate leaf-specific expression at the appropriate developmental stages. Deletion analyses of the promoter region indicated that regulatory elements determining vascular cell-specific expression are present in the 200-bp region between -245 bp and -45 bp from the transcription initiation site. By transforming the Hd3a-GUS construct to rice cultivar ‘Taichung 65’ which is defective in Ehd1, we observed that Ehd1 is the major regulatory element that controls Hd3a promoter activity.
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등록된 정보가 없습니다.
참고문헌 (62)
참고문헌 신청
Adrian, J. / 2010 / Cis-regulatory elements and chromatin state coordinately control temporal and spatial expression of FLOWERING LOCUS T in Arabidopsis / Plant Cell 22:1425~1440
Alsterfjord, M. / 2004 / Plasma membrane $H^+$ -ATPase and 14-3-3 isoforms of Arabidopsis leaves: evidence for isoform specificity in the 14-3-3/$H^+$ -ATPase interaction / Plant Cell Physiol 45:1202~1210
An, G. / 1989 / Binary vectors. In Plant Molecular Biology Manual, A3 / Kluwer Academic Publisher :1~19
An, H.L. / 2004 / CONSTANS acts in the phloem to regulate a systemic signal that induces photoperiodic flowering of Arabidopsis / Development 131:3615~3626
Asano, T. / 2002 / Rpp16 and Rpp17, from a common origin, have different protein characteristics but both genes are predominantly expressed in rice phloem tissues / Plant Cell Physiol. 43:668~674
Alsterfjord, M. / 2004 / Plasma membrane
An, G. / 1989 / Binary vectors. In Plant Molecular Biology Manual, A3 / Kluwer Academic Publisher :1~19
An, H.L. / 2004 / CONSTANS acts in the phloem to regulate a systemic signal that induces photoperiodic flowering of Arabidopsis / Development 131:3615~3626
Asano, T. / 2002 / Rpp16 and Rpp17, from a common origin, have different protein characteristics but both genes are predominantly expressed in rice phloem tissues / Plant Cell Physiol. 43:668~674
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