Ultrastructural changes in cristae of lymphoblasts in acute lymphoblastic leukemia parallel alterations in biogenesis markers

Ritika Singh; Ritika Singh; Jayanth Kumar Palanichamy; T. C. Nag; Sameer Bakhshi; Archna Singh

doi:10.1186/s42649-021-00069-4

추천

검색

자료유형: 학술저널

저자정보: Ritika Singh (All India Institute of Medical Sciences) Ritika Singh (All India Institute of Medical Sciences) Jayanth Kumar Palanichamy (All India Institute of Medical Sciences) T. C. Nag (All India Institute of Medical Sciences) Sameer Bakhshi (All India Institute of Medical Sciences) Archna Singh (All India Institute of Medical Sciences)

저널정보: 한국현미경학회 한국현미경학회지 한국현미경학회지 제51권 제4호

발행연도: 2021.12

수록면: 1 - 12 (12page)

DOI: 10.1186/s42649-021-00069-4

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

We explored the link between mitochondrial biogenesis and mitochondrial morphology using transmission electron microscopy (TEM) in lymphoblasts of pediatric acute lymphoblastic leukemia (ALL) patients and compared these characteristics between tumors and control samples. Gene expression of mitochondrial biogenesis markers was analysed in 23 ALL patients and 18 controls and TEM for morphology analysis was done in 15 ALL patients and 9 healthy controls. The area occupied by mitochondria per cell and the cristae cross-sectional area was observed to be significantly higher in patients than in controls ( p -value?=?0.0468 and p -value<?0.0001, respectively). The mtDNA copy numbers, TFAM , POLG, and c-myc gene expression were significantly higher in ALL patients than controls (all p -values<?0.01). Gene Expression of PGC-1α was higher in tumor samples. The analysis of the correlation between PGC-1α expression and morphology parameters i.e., both M/C ratio and cristae cross-sectional area revealed a positive trend ( r =?0.3, p =?0.1). The increased area occupied by mitochondria and increased cristae area support the occurrence of cristae remodelling in ALL. These changes might reflect alterations in cristae dynamics to support the metabolic state of the cells by forming a more condensed network. Ultrastructural imaging can be useful for affirming changes occurring at a subcellular organellar level.

참고문헌 (58)

참고문헌 신청

E. Alirol / 2006 / Mitochondria and cancer: Is there a morphological connection? / Oncogene 25:4706

S. Andrzejewski / 2017 / PGC-1α promotes breast cancer metastasis and confers bioenergetic flexibility against metabolic drugs / Cell Metab 26:778

G. Arismendi-Morillo / 2009 / Electron microscopy morphology of the mitochondrial network in human cancer / Int. J. Biochem. Cell Biol 41:2062

R. S. Arora / 2016 / Acute leukemia in children: A review of the current Indian data / South Asian J. Cancer 5:155

N. Baker / 2019 / Linking mitochondrial dynamics, cristae remodeling and supercomplex formation: How mitochondrial structure can regulate bioenergetics / Mitochondrion 49:259

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!