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학술저널
저자정보
김세연 (부산대학교) 우동협 (부산대학교 치의학전문대학원) 이민아 (부산대학교 치의학전문대학원 예방과사회치의학교실) 김지수 (부산대학교) 이정하 (부산대학교) 정승화 (부산대학교)
저널정보
대한예방치과·구강보건학회 대한구강보건학회지 대한구강보건학회지 제41권 제1호
발행연도
2017.3
수록면
22 - 27 (6page)

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Objectives: Dental plaque is composed of 700 bacterial species. It is known that some oral microorganisms produce porphyrin, and thus, they emit red fluorescence when illuminated with blue light at a specific wavelength of <410 nm. Porphyromonas gingivalis belongs to the genus Porphyromonas , which is characterized by the production of porphyrin. The aim of this study was to evaluate red fluorescence emission of some oral microorganisms interacting with P. gingivalis . Methods: Five bacterial strains ( P. gingivalis , Streptococcus mutans , Lactobacillus casei , Actinomyces naeslundii , and Fusobacterium nucleatum ) were used for this study. Tryptic soy agar medium supplemented with hemin, vitamin K3, and sheep blood was used as a growth medium. The fluorescence emission of bacterial colonies was evaluated under 405 nm-wavelength blue light using a Quantitative Light-induced Fluorescence Digital (QLF-D) camera system. Each bacterium was cultured alone and cocultured in close proximity with P. gingivalis . The red/green (R/G) ratio of fluorescence image was calculated and the differences of R/G ratio according to each growth condition were compared using the Mann-Whitney test ( P <0.05). Results: Single cultured S. mutans , L. casei and A. naeslundii colonies emitted red fluorescence (R/ G ratio=2.15±0.06, 4.31±0.17, 5.52±1.29, respectively). Fusobacterium nucleatum colonies emitted green fluorescence (R/G ratio=1.36±0.06). The R/G ratios of A. naeslundii and F. nucleatum were increased when P. gingivalis was co-cultured with each bacterium ( P <0.05). In contrast, the R/G ratios of S. mutans and L. casei were decreased when P. gingivalis was co-cultured with each bacterium ( P =0.002, 0.003). Conclusions: This study confirmed that P. gingivalis could affect the red fluorescence of other oral bacteria under 405 nm-wavelength blue light. Our findings concluded that P. gingivalis has an important role for red fluorescence emission of dental biofilm.

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