지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한약리학회 The Korean Journal of Physiology & Pharmacology The Korean Journal of Physiology & Pharmacology 제22권 제3호
- 발행연도
- 2018.5
- 수록면
- 349 - 360 (12page)
- DOI
- https://doi.org/10.4196/kjpp.2018.22.3.349
이용수
초록· 키워드
오류제보하기
Autophagy has been studied as a therapeutic strategy for cardiovascular diseases. However, insufficient studies have been reported concerning the influence of vascular smooth muscle cells (VSMCs) through autophagy regulation. The aim of the present study was to determine the effects of VSMCs on the regulation of autophagy under in vitro conditions similar to vascular status of the equipped microtubule target agent-eluting stent and increased release of platelet-derived growth factor-BB (PDGF-BB). Cell viability and proliferation were measured using MTT and cell counting assays. Immunofluorescence using an anti-α-tubulin antibody was performed to determine microtubule dynamic formation. Cell apoptosis was measured by cleavage of caspase-3 using western blot analysis, and by nuclear fragmentation using a fluorescence assay. Autophagy activity was assessed by microtubule-associated protein light chain 3-II (LC-II) using western blot analysis. Levels of intracellular reactive oxygen species (ROS) were measured using H2DCFDA. The proliferation and viability of VSMCs were inhibited by microtubule regulation. Additionally, microtubule- regulated and PDGF-BB-stimulated VSMCs increased the cleavage of caspase- 3 more than only the microtubule-regulated condition, similar to that of LC3- II, implying autophagy. Inhibitory autophagy of microtubule-regulated and PDGFBB- stimulated VSMCs resulted in low viability. However, enhancement of autophagy maintained survival through the reduction of ROS. These results suggest that the apoptosis of conditioned VSMCs is decreased by the blocking generation of ROS via the promotion of autophagy, and proliferation is also inhibited. Thus, promoting autophagy as a therapeutic target for vascular restenosis and atherosclerosis may be a good strategy.
목차
등록된 정보가 없습니다.
참고문헌 (51)
참고문헌 신청
Chistiakov DA / 2015 / Vascular smooth muscle cell in atherosclerosis / Acta Physiol (Oxf ) 214:33~50
Shioi A / 2018 / Plaque calcification during atherosclerosis progression and regression / J Atheroscler Thromb 25:294~303
van der Wal AC / 1999 / Atherosclerotic plaque rupture-pathologic basis of plaque stability and instability / Cardiovasc Res 41:334~344
Ramji DP / 2015 / Cytokines in atherosclerosis: key players in all stages of disease and promising therapeutic targets / Cytokine Growth Factor Rev 26:673~685
Ross R / 1987 / Growth factors in the pathogenesis of atherosclerosis / Acta Med Scand Suppl 715:33~38
Shioi A / 2018 / Plaque calcification during atherosclerosis progression and regression / J Atheroscler Thromb 25:294~303
van der Wal AC / 1999 / Atherosclerotic plaque rupture-pathologic basis of plaque stability and instability / Cardiovasc Res 41:334~344
Ramji DP / 2015 / Cytokines in atherosclerosis: key players in all stages of disease and promising therapeutic targets / Cytokine Growth Factor Rev 26:673~685
Ross R / 1987 / Growth factors in the pathogenesis of atherosclerosis / Acta Med Scand Suppl 715:33~38
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Autophagy in the placenta
Obstetrics & Gynecology Science
2017 .01
-
Autophagy in Tumorigenesis and Cancer Treatment
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Autophagy and its regulation by ginseng components
Journal of Ginseng Research
2019 .06
-
Radiation-Induced Autophagy Contributes to Cell Death and Induces Apoptosis Partly in Malignant Glioma Cells
Cancer Research and Treatment
2015 .01
-
Role of Autophagy in the Pathogenesis of Diabetes and Therapeutic Potential of Autophagy Modulators in the Treatment of Diabetes and Metabolic Syndrome
Journal of Korean Medical Science
2022 .09
-
Therapeutic targets and drugs for hyper-proliferation of vascular smooth muscle cells
Journal of Pharmaceutical Investigation
2020 .01
-
Induction of Forkhead Class box O3a and apoptosis by a standardized ginsenoside formulation, KG-135, is potentiated by autophagy blockade in A549 human lung cancer cells
Journal of Ginseng Research
2017 .07
-
Autophagy Inhibition Promotes Quercetin Induced Apoptosis in MG-63 Human Osteosarcoma cells
International Journal of Oral Biology
2015 .01
-
Autophagy: a lysosomal degradation process for cellular homeostasis and its relationship with oral squamous cell carcinoma
International Journal of Oral Biology
2021 .01
-
Sensitization of hepatocellular carcinoma cells against sorafenib treatment with autophagy regulation
한국실험동물학회 학술발표대회 논문집
2019 .07
-
Remifentanil induces autophagy and prevents hydrogen peroxide-induced apoptosis in Cos-7 cells
Journal of dental anesthesia and pain medicine
2016 .01
-
DE21 inhibits PDGF-induced proliferation of rat aortic vascular smooth muscle cell by up-regulation of p53 leading to G1/S cell cycle arrest
한국실험동물학회 학술발표대회 논문집
2015 .02
-
Curcumin-Induced Autophagy Augments Its Antitumor Effect against A172 Human Glioblastoma Cells
Biomolecules & Therapeutics(구 응용약물학회지)
2019 .01
-
Autophagy in neutrophils
The Korean Journal of Physiology & Pharmacology
2020 .01
-
De novo design of a novel AIE fluorescent probe tailored to autophagy visualization via pH manipulation
생체재료학회지
2023 .03
-
Can Cancer Therapy be Achieved by Bridging Apoptosis and Autophagy: a Method Based on microRNA-Dependent Gene Therapy and Phytochemical Targets
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Autophagy localization and cytoprotective role in cisplatin-induced acute kidney injury
Korean Journal of Veterinary Research(대한수의학회지)
2019 .10
-
Novel functional roles of caspase-related genes in the regulation of apoptosis and autophagy
The Korean Journal of Physiology & Pharmacology
2016 .11
-
Rutin induces autophagy in cancer cells
International Journal of Oral Biology
2016 .01
-
Propofol protects human keratinocytes from oxidative stress via autophagy expression
Journal of dental anesthesia and pain medicine
2017 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0