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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.8
- 수록면
- 865 - 874 (10page)
- DOI
- https://doi.org/10.3803/EnM.2021.1108
이용수
초록· 키워드
오류제보하기
Background: Despite clinical evidence indicating poor muscle health in subjects with primary aldosteronism (PA), it is still unclearwhether the role of aldosterone in muscle metabolism is direct or mediated indirectly via factors, such as electrolyte imbalance orimpaired glucose uptake. As one approach to clarify this issue, we investigated the effect of aldosterone on in vitro myogenesis andthe potential mechanism explaining it.
Methods: Myogenesis was induced in mouse C2C12 myoblasts with 2% horse serum. Immunofluorescence, quantitative reversetranscription polymerase chain reaction, Western blot, viability, and migration analyses were performed for experimental research.
Results: Recombinant aldosterone treatment suppressed muscle differentiation from mouse C2C12 myoblasts in a dose-dependentmanner, and consistently reduced the expression of myogenic differentiation markers. Furthermore, aldosterone significantly increased intracellular reactive oxygen species (ROS) levels in myotubes, and treatment with N-acetyl cysteine, a potent biological thiol antioxidant, reversed the decrease of myotube area, myotube area per myotube, nucleus number per myotube, and fusion index dueto aldosterone through decreasing oxidative stress. A binding enzyme-linked immunosorbent assay confirmed that mineralocorticoidreceptor (MR) interacted with aldosterone in C2C12 myoblasts, while eplerenone, an MR inhibitor, blocked aldosterone-stimulatedintracellular ROS generation during myogenesis and markedly attenuated the suppression of in vitro myogenesis by aldosterone.
Conclusion: These findings support the hypothesis that hypersecretion of aldosterone, like PA, directly contributes to muscular deterioration and suggest that antioxidants and/or MR antagonists could be effective therapeutic options to reduce the risk of sarcopeniain these patients.
목차
등록된 정보가 없습니다.
참고문헌 (8)
참고문헌 신청
탐 다오 / 2020 / Sarcopenia and Muscle Aging: A Brief Overview / Endocrinology and Metabolism 35(4):716~732
김유근 / 2018 / Effect of Sarcopenia on Postoperative Mortality in Osteoporotic Hip Fracture Patients / 대한골대사학회지 25(4):227~233
김상훈 / 2019 / Sarcopenia Associated with Chronic Obstructive Pulmonary Disease / 대한골대사학회지 26(2):65~74
Sergei G. Tevosian / 2019 / Molecular Mechanisms of Primary Aldosteronism / Endocrinology and Metabolism 34(4):355~366
Zheng-Wei Chen / 2018 / Primary Aldosteronism and Cerebrovascular Diseases / Endocrinology and Metabolism 33(4):429~434
김유근 / 2018 / Effect of Sarcopenia on Postoperative Mortality in Osteoporotic Hip Fracture Patients / 대한골대사학회지 25(4):227~233
김상훈 / 2019 / Sarcopenia Associated with Chronic Obstructive Pulmonary Disease / 대한골대사학회지 26(2):65~74
Sergei G. Tevosian / 2019 / Molecular Mechanisms of Primary Aldosteronism / Endocrinology and Metabolism 34(4):355~366
Zheng-Wei Chen / 2018 / Primary Aldosteronism and Cerebrovascular Diseases / Endocrinology and Metabolism 33(4):429~434
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