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자료유형
학술저널
저자정보
김규태 (Department of Applied Plant Science Chonnam National University Korea) 박아론 (Department of Applied Plant Science Chonnam National University Korea) 김운지 (Department of Applied Plant Science Chonnam National University Korea) 문창역 (Department of Applied Plant Science Chonnam National University Korea) 강병희 (Department of Applied Plant Science Chonnam National University Korea) 김성훈 (National Agrobiodiversity Center National Institute of Agricultural Sciences RDA Korea) 최유미 (National Agrobiodiversity Center National Institute of Agricultural Sciences RDA Korea) 하보근 (Department of Applied Plant Science Chonnam National University Korea)
저널정보
한국육종학회 Plant Breeding and Biotechnology Plant Breeding and Biotechnology Vol.9 No.3
발행연도
2021.9
수록면
250 - 258 (9page)

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The soluble sugar content of legume seeds affects the final flavor of the legume and legume products. The purpose of this study was to develop a rapid, simple, and low-cost colorimetric method for high-throughput screening of sucrose content in legume seeds. This colorimetric method was based on the enzymatic reactions of invertase (INV) and glucose oxidase (GOD). Briefly, 20 different soybean and cowpea varieties were used in this study. For sucrose extraction, solvent-to-sample ratios of 10:1 and 5:1 were found to result in optimal absorbance values for determining sucrose content in soybean and cowpea, respectively. The extraction efficiency was also evaluated under various extraction temperatures (25℃ and 50℃) and incubation times (15 minutes, 2 hours, 8 hours, and 24 hours) and the sucrose content was found to increase with increasing temperature and time. Accordingly, the optimal extraction conditions were 24 hours of incubation at 50℃. Under this condition, the GOD/INV method had significant positive correlations (r = 0.91** for soybean and r = 0.87** for cowpea) with the high-performance liquid chromatography method. Overall, this colorimetric method is a fast, simple, and inexpensive tool for quantitative determination of sucrose content in legumes.

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