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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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The redox-sensing repressor Rex is a homodimeric transcriptional regulator involved in expression of respiratory genes.
Because nicotinamide adenine dinucleotide (NAD) exists oxidized or reduced form by catabolic metabolism, intracellularNAD+/NADH ratio can be a key signal indicating the cellular redox state. The Rex from hyperthermophilic bacterium,Thermotoga maritima (TmRex), was cloned and overexpressed in Escherichia coli. The TmRex is composed of 208amino-acid residues with a molecular mass of 22,954 Da. The TmRex crystals were obtained by the sitting-drop vapourdiffusionmethod and diffracted to 1.95 A resolution. The crystals belonged to the monoclinic space group P21, with unitcellparameters a = 53.54 A, b = 88.34 A, c = 87.84 A, and β = 96.74°. Two dimeric molecules of TmRex were present in anasymmetric unit, giving a solvent contents of 45.17%.
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참고문헌 (10)
참고문헌 신청
Belenky, P / 2007 / NAD+ metabolism in health and disease / Trends Biochem Sci 32:12~19
Brekasis, D / 2003 / A novel sensor of NADH/NAD+ redox poise in Streptomyces coelicolor A3(2) / EMBO J 22:4856~4865
Giaccia, A. J / 2004 / The biology of hypoxia : the role of oxygen sensing in development, normal function, and disease / Genes Dev 18:2183~2194
Gyan, S / 2006 / Regulatory loop between redox sensing of the NADH/NAD(+)ratio by Rex(YdiH)and oxidation of NADH by NADH dehydrogenase Ndh in Bacillus subtilis / J Bacteriol 188:7062~7071
McCoy, A. J / 2007 / Phaser crystallographic software / J Appl Crystallogr 40:658~674
Brekasis, D / 2003 / A novel sensor of NADH/NAD+ redox poise in Streptomyces coelicolor A3(2) / EMBO J 22:4856~4865
Giaccia, A. J / 2004 / The biology of hypoxia : the role of oxygen sensing in development, normal function, and disease / Genes Dev 18:2183~2194
Gyan, S / 2006 / Regulatory loop between redox sensing of the NADH/NAD(+)ratio by Rex(YdiH)and oxidation of NADH by NADH dehydrogenase Ndh in Bacillus subtilis / J Bacteriol 188:7062~7071
McCoy, A. J / 2007 / Phaser crystallographic software / J Appl Crystallogr 40:658~674
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