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Pseudomonas aeruginosa is an opportunistic pathogen, and have multiple multidrug efflux pumps. The MexEF-OprNmultidrug efflux system is overexpressed in nfxC-type mutants and give resistance to quinolones, chloramphenicol andtrimethoprim. A LysR-type transcriptional activator, MexT, is the major activator of this efflux system. Although the activityof MexT is regulated in response to the cellular redox state, the ligand and activation mechanism remain unknown yet.
MexT consists of the N-terminal DNA binding domain and the C-terminal regulatory domain that contains the ligandbinding site. In this study, we overproduced and purified the regulatory domain of MexT from P. aeruginosa and obtainedthe crystals suitable for the structural study. The crystal diffracted X-ray to 2.3 A resolution, revealing that the crystalsbelong to space group P212121, with unit cell parameters a = 65.7, b = 108.5, and c = 109.1 A. The cell content analysissuggested that 3 or 4 molecules are contained in the asymmetric unit. To solve the phasing problem, growing of the Se-Met substituted crystals are underway. This structure will give insight into the molecular mechanism of the activation ofMexT in the presence of antibiotics.
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참고문헌 (14)
참고문헌 신청
Devesse, L / 2011 / Crystal structures of DntR inducer binding domains in complex with salicylate offer insights into the activation of LysR-type transcriptional regulators / Mol Microbiol 81:354~367
Fargier, E / 2012 / MexT functions as a redox-responsive regulator modulating disulfide stress resistance in Pseudomonas aeruginosa / J Bacteriol 194:3502~3511
장용대 / 2018 / A Novel Tetrameric Assembly Configuration in VV2_1132, a LysR-Type Transcriptional Regulator in Vibrio vulnificus / Molecules and Cells 41(4):301~310
Jeong, H / 2016 / Pseudoatomic Structure of the Tripartite Multidrug Efflux Pump AcrAB-TolC Reveals the Intermeshing Cogwheel-like Interaction between AcrA and TolC / Structure 24:272~276
Jo, I / 2015 / Structural details of the OxyR peroxide-sensing mechanism / Proc Natl Acad Sci U S A 112:6443~6448
Fargier, E / 2012 / MexT functions as a redox-responsive regulator modulating disulfide stress resistance in Pseudomonas aeruginosa / J Bacteriol 194:3502~3511
장용대 / 2018 / A Novel Tetrameric Assembly Configuration in VV2_1132, a LysR-Type Transcriptional Regulator in Vibrio vulnificus / Molecules and Cells 41(4):301~310
Jeong, H / 2016 / Pseudoatomic Structure of the Tripartite Multidrug Efflux Pump AcrAB-TolC Reveals the Intermeshing Cogwheel-like Interaction between AcrA and TolC / Structure 24:272~276
Jo, I / 2015 / Structural details of the OxyR peroxide-sensing mechanism / Proc Natl Acad Sci U S A 112:6443~6448
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