지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.2
- 수록면
- 63 - 69 (7page)
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We identified a peel-specific expressed gene in Citrus unshiu fruits by differentially expressed gene (DEG) analysis, which showed a homology with carotenoid isomerase-like genes identified from other plants and, therefore, designated as CuCRTISO-like. Here we determined the promoter sequence of CuCRTISO-like and analyzed histochemical GUS activity using transgenic Arabidopsis plants harboring CuCRTISO-like promoter-GUS gene constructs (pCRTL-Prom1~pCRTL-Prom5 lines).
The promoter activity of CuCRTISO-like was detected in the cotyledon at 5 and 10 days after germination (DAG), young leaf, and anther, but not in the cotyledon at 15 DAG and mature leaf. Several cis-acting elements involved in hormones and abiotic stresses are located on the CuCRTISO- like promoter. Salicylic acid and ethylene treatments induced the GUS activity in the pCRTL-prom1 and pCRTL-Prom4 line, respectively. Treatment of drought and wounding stress induced the GUS activity in the pCRTLProm4 and pCRTL-Prom3 line, respectively. Heat stress treatment induced GUS activity more strongly as the promoter length decreased except for no GUS activity in the pCRTL-Prom5 line. The CuCRTISO-like expression during fruit maturation of C. unshiu showed a peel-specific expression pattern. Our results suggest that CuCRTISOlike promoter activity is regulated in a developmental and organ-specific manner, and responds to hormones and abiotic stresses.
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참고문헌 (36)
참고문헌 신청
Amirimani B / 2003 / Increased transcriptional activity of the CYP3A4*1B promoter variant / Environ and Mol Mut 42:299~305
Baranowskij N / 1994 / A novel DNA binding protein with homology to Myb oncoproteins containing only one repeat can function as a transcriptional activator / EMBO J 13:5383~5392
Bramley PM / 2002 / Regulation of carotenoid formation during tomato fruit ripening and development / J Exp Bot 53:2107~2113
Cao X / 2007 / Abscisic acid and stress signals induce Viviparous1 expression in seed and vegetative tissues of maize / Plant Physiol 143:720~731
Cazzonelli CI / 2009 / Regulation of carotenoid composition and shoot branching in Arabidopsis by a chromatin modifying histone methyltransferase, SDG8 / Plant Cell 21:39~53
Baranowskij N / 1994 / A novel DNA binding protein with homology to Myb oncoproteins containing only one repeat can function as a transcriptional activator / EMBO J 13:5383~5392
Bramley PM / 2002 / Regulation of carotenoid formation during tomato fruit ripening and development / J Exp Bot 53:2107~2113
Cao X / 2007 / Abscisic acid and stress signals induce Viviparous1 expression in seed and vegetative tissues of maize / Plant Physiol 143:720~731
Cazzonelli CI / 2009 / Regulation of carotenoid composition and shoot branching in Arabidopsis by a chromatin modifying histone methyltransferase, SDG8 / Plant Cell 21:39~53
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