지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Promoters are essential regulatory elements for efficiently expressing a gene of interest in a target tissue of a organism. Therefore, the identification of a suitable promoter is important in plant biotechnology. In this study, four promoters were selected and identified to be constitutively or tissue-specifically expressed in Brassica rapa bacterial artificial chromosome (BAC) clones using the results of transcriptomic analysis of Brassica rapa and open-source database information on Arabidopsis thaliana. The 2 kb region of the 5′ upstream was isolated from the Brassica rapa genomic DNA for each promoter. The four promoters were then fused to β-glucuronidase (gus) and green fluorescent protein (gfp) genes, and the recombinant transgenes were introduced into Arabidopsis. As a result of histochemical GUS staining, GFP fluorescence and RT-PCR, the gus gene was observed to be expressed constitutively in all tissues using all four promoters. A GUS activity assay using fluorescent 4-MUG revealed that the BR11 promoter showed similar activity to the CaMV35S promoter, while the BR4, BR15, and BR16 promoters showed 1.5, 4, and 18-fold higher activities than the CaMV35S promoter, respectively. These results indicate that these four promoters could be used to incorporate useful genes with enhanced function into crops of interest.
목차
등록된 정보가 없습니다.
참고문헌 (42)
참고문헌 신청
Al-Kaff NS / 1998 / Transcriptional and post-transcriptional gene silencing in response to a pathogen / Science 279:2113~2115
An YQ / 1996 / Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues / Plant J 10:107~121
Brugliera F / 2013 / Violet/blue chrysanthemums-metabolic engineering of the anthocyanin biosynthetic pathway results in novel petal colors / Plant Cell Physiol 54:1696~1710
Cai M / 2007 / A rice promoter containing both novel positive and negative cis-elements for regulation of green tissue-specific gene expression in transgenic plants / Plant Biotechnol J 5:664~674
Callis J / 1990 / Structure, localization, and expression of their promoters in transgenic tobacco / J Biol Chem 265:12486~12493
An YQ / 1996 / Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues / Plant J 10:107~121
Brugliera F / 2013 / Violet/blue chrysanthemums-metabolic engineering of the anthocyanin biosynthetic pathway results in novel petal colors / Plant Cell Physiol 54:1696~1710
Cai M / 2007 / A rice promoter containing both novel positive and negative cis-elements for regulation of green tissue-specific gene expression in transgenic plants / Plant Biotechnol J 5:664~674
Callis J / 1990 / Structure, localization, and expression of their promoters in transgenic tobacco / J Biol Chem 265:12486~12493
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