지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.6
- 수록면
- 161 - 169 (9page)
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초록· 키워드
오류제보하기
Agroinoculation transient expression systems are commonly accompanied with elevated pathogenesisrelated (PR) protein production and leaf necrosis. We identified the major PR proteins in Nicotiana benthamiana in response to agroinoculation and determined that their occurrence was mainly due to agrobacterium infection and the method of inoculation, rather than due to viral vectors overexpressing foreign proteins. A spray-on inoculation method was optimized in this research and used to obviate the leaf necrosis and PR proteins induced by agrobacterium.
Subsequently, this method also increased the yield and purity of the protein-of-interest. A further investigation of PR protein induction by a necrosis-inducing protein, Jun a 1, suggested that the plant pathogenic response was related to biochemical integrity of plant cell wall, which was also confirmed by an osmo-stabilizing mannitol ex vivo culture experiment. These findings provide insight into the response of plants to agroinoculation and suggested a connection between cell wall weakening and PR protein elicitation.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Bayley ST / 1957 / The influence of mannitol and auxin on growth of cell walls in avena coleoptiles / Ann Bot 21:633~641
Bent AF / 2000 / Arabidopsis in planta transformation. Uses, mechanisms, and prospects for transformation of other species / Plant Physiol 124:1540~1547
변의백 / 2015 / β-(1,3)-Glucan Isolated from Agrobacterium Species Induces Maturation of Bone Marrow-derived Dendritic Cells and Drives Th1 Immune Responses / Food Science and Biotechnology 24(4):1533~1540
Collmer A / 1990 / Pectic enzyme production and bacterial plant pathogenicity / Advances in molecular genetics of plant-microbe interactions vol 1: proceedings of the 5th international symposium on the molecular genetics of plant-microbe interactions 1:65~72
Cornelissen BJC / 1986 / A tobacco mosaic virus-induced tobacco protein is homologous to the sweet-tasting protein thaumatin / Nature 321:531~532
Bent AF / 2000 / Arabidopsis in planta transformation. Uses, mechanisms, and prospects for transformation of other species / Plant Physiol 124:1540~1547
변의백 / 2015 / β-(1,3)-Glucan Isolated from Agrobacterium Species Induces Maturation of Bone Marrow-derived Dendritic Cells and Drives Th1 Immune Responses / Food Science and Biotechnology 24(4):1533~1540
Collmer A / 1990 / Pectic enzyme production and bacterial plant pathogenicity / Advances in molecular genetics of plant-microbe interactions vol 1: proceedings of the 5th international symposium on the molecular genetics of plant-microbe interactions 1:65~72
Cornelissen BJC / 1986 / A tobacco mosaic virus-induced tobacco protein is homologous to the sweet-tasting protein thaumatin / Nature 321:531~532
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