Expression in rice of an autoactive variant of Medicago truncatula DMI3, the Ca+2/calmodulin-dependent protein kinase from the common symbiotic pathway modifies root transcriptome and improves mycorrhizal colonization

Marlene Ortiz-Berrocal; Luis Lozano; Alejandro Sanchez-Flores; Noreide Nava; Georgina Herna´ndez; Pallavolu M. Reddy

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자료유형: 학술저널

저자정보: Marlene Ortiz-Berrocal (Universidad Nacional Auto´noma de Me´xico) Luis Lozano (Universidad Nacional Auto´noma de Me´xico) Alejandro Sanchez-Flores (Universidad Nacional Auto´noma de Me´xico) Noreide Nava (Universidad Nacional Auto´noma de Me´xico) Georgina Herna´ndez (Universidad Nacional Auto´noma de Me´xico) Pallavolu M. Reddy (Universidad Nacional Auto´noma de Me´xico)

저널정보: 한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제11권 제5호

발행연도: 2017.10

수록면: 271 - 287 (17page)

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Rice is the principle staple food for more than half of humankind. Frequently, productivity of rice is affected by low nitrogen in the soil and hence, for enhanced rice production it heavily relies on synthetic nitrogen fertilizers that beget economic and ecological costs. In this context, the interest in transferring legumelike biological nitrogen fixation capability to rice has increased lately. The rice-arbuscular mycorrhizal (AM) symbiosis is mediated by genes that are orthologous to legume-genes known to be essential constituents of the common symbiotic pathway (CSP) that facilitates the establishment of both rhizobial nitrogen fixation- and AMsymbioses in legumes. Particularly, DMI3 (Ca?2/- calmodulin-dependent serine/threonine protein kinase, CCaMK), a component of the CSP, was found to play a paramount role in promoting the development of both types of symbioses. In fact, expression of autoactive version of DMI3 was shown to be sufficient to trigger downstream developmental processes leading to the induction of spontaneous nodulation in the absence of rhizobia. Hence, in the present investigation, we expressed in transgenic rice a gain-of-function Medicago truncatula DMI3T271D gene (gofMtDMI3) and assessed if legume-like symbiotic responses can be mimicked in rice roots. Ectopic expression of gofMtDMI3 in common bean induced spontaneous nodulation in the roots in the absence of rhizobia, but in rice plants it did not produce any such legume-like nodular manifestations. Conversely, the expression of gofMtDMI3 supported elevated AM colonization in rice roots that could improve plant nutrition/growth. In addition, gofMtDMI3 expression induced higher transcript levels of the CSP orthologues OsDMI3, OsIPD3 and OsNSP1, as well as triggered changes in the expression of several genes involved in biotic and abiotic stress responses. Our results with gofMtDMI3 lay the basis for the potential development of a biotechnological approach towards improvement of rice production.

#Rice #Legumes #Common symbiotic pathway (CSP) #Medicago truncatula DMI3 (CCaMK) #AM symbiosis #Transcriptome analysis

참고문헌 (90)

참고문헌 신청

Kohki Akiyama / 2005 / Plant sesquiterpenes induce hyphal branching in arbuscular mycorrhizal fungi / Nature 435(7043):824~827

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