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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.10
- 수록면
- 315 - 329 (15page)
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초록· 키워드
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The expression of insecticidal genes must be induced at appropriate time and in sufficient amount to confer protection against targeted pests. However, the increased scientific reports of resistance development in insect pest against insecticidal delta-endotoxins, produced by Bacillus thuringiensis, provide impetus for the development of alternative insect management strategies. The present study was conducted to investigate the importance of targeted expression of a hybrid insecticidal gene (SN19) in potatoes. For this purpose, two plant expression vectors were constructed by cloning hybrid SN19 gene (cry1Badomain I?III and cry1Ia-domain II) under the control of a wound-inducible promoter isolated from Asparagus officinalis (AoPR1) and CaMV 35S promoter, and were transferred to Agrobacterium tumefaciens strain EHA 105. Four potato genotypes (Marabel, Innovator, Tokat 10/1 and Tokat 6/24) were transformed with EHA 105 strain harboring pTF101.1 35S?SN19 and pTF101.1 AoPR1?SN19 constructs. Phosphinothricin (PPT) was used at concentration of 1 mg/l for selection of primary transformants.
PCR results showed the presence of both introduced SN19 and bar genes in 43 plants out of total 154 putative transgenics. Expression of SN19 protein in primary transformants was confirmed by Western blot assays. The mechanical wounding of transgenic plants exhibited more accumulated levels of SN19 proteins during post wounding period. Leaf biotoxicity assays with Colorado potato beetle (Coleoptera) and tomato leafminer (Lepidoptera) exhibited 100% mortality of the pests in primary transformants.
Based on our mortality results with both constructs, we concluded that the potato transgenic lines exhibited targeted expression of insecticidal gene under the control of AoPR1 promoter upon insect wounding with eliminated toxicity of Cry protein and hence can be further used effectively in potato breeding programme.
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참고문헌 (48)
참고문헌 신청
Aasim M / 2010 / In vitro shoot regeneration of NAA-pulse treated plumular leaf explants of cowpea / Not Sci Biol 2:60~63
Aasim M / 2010 / Efficient in vitro propagation from preconditioned embryonic axes of Turkish cowpea (Vigna unguiculata L.) cultivar Akkiz / Arch Biol Sci 62:1047~1052
Adamczyk JJ / 2009 / Production of mRNA from the Cry1Ac transgene differs among bollgard lines which correlate to the level of subsequent protein / Transgenic Res 18:143~149
Alyokhin A / 2008 / Colorado potato beetle resistance to insecticides / Am J Potato Res 85:395~413
Amarasinghe BHR / 2006 / Genomic approaches to the discovery of promoters for sustained expression in cotton (Gossypium hirsutum L.) under field conditions: expression analysis in transgenic cotton and Arabidopsis of a RuBisCo small subunit promoter identified using EST sequence analysis and cDNA microarrays / Plant Biotechnol 23:437~450
Aasim M / 2010 / Efficient in vitro propagation from preconditioned embryonic axes of Turkish cowpea (Vigna unguiculata L.) cultivar Akkiz / Arch Biol Sci 62:1047~1052
Adamczyk JJ / 2009 / Production of mRNA from the Cry1Ac transgene differs among bollgard lines which correlate to the level of subsequent protein / Transgenic Res 18:143~149
Alyokhin A / 2008 / Colorado potato beetle resistance to insecticides / Am J Potato Res 85:395~413
Amarasinghe BHR / 2006 / Genomic approaches to the discovery of promoters for sustained expression in cotton (Gossypium hirsutum L.) under field conditions: expression analysis in transgenic cotton and Arabidopsis of a RuBisCo small subunit promoter identified using EST sequence analysis and cDNA microarrays / Plant Biotechnol 23:437~450
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