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논문 기본 정보

자료유형
학술저널
저자정보
Amir Hameed (National Institute for Biotechnology and Genetic Engineering) Rakhshanda Bilal (National Institute for Biotechnology and Genetic Engineering) Farooq Latif (National Institute for Biotechnology and Genetic Engineering) Joyce Van Eck (Boyce Thompson Institute for Plant Research) Georg Jander (Boyce Thompson Institute for Plant Research) Shahid Mansoor (National Institute for Biotechnology and Genetic Engineering)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제12권 제3호
발행연도
2018.6
수록면
175 - 185 (11page)

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Potato tubers must be cold-stored to extend their shelf life and maintain an uninterrupted supply chain for food processors. However, a side-effect of low-temperature storage is manifested in terms of cold-induced sweetening (CIS) of potato tubers, which reduces the processing quality and the commercial value of the end-products. RNA interference (RNAi) technology, whereby transgene-derived small interfering RNAs can trigger the homology-based knockdown of cognate host genes and can initiate gene silencing, has been successfully applied in crop improvement through targeted gene knockout in host plants. In the current study, transgenic potato plants (Solanum tuberosum cv. Desiree) were generated, expressing a 300 bp hairpin loop nucleotide sequence targeting the potato vacuolar invertase gene (VInv), under the constitutive Cauliflower mosaic virus 35S promoter. Tubers collected from transgenic lines showed a significant reduction in reducing sugar content after 180 days of cold storage, without showing any measurable off-target effects on plant morphology and tuberization compared to nontransformed control plants. The cold-stored tubers were further assayed for chip color, which showed a fairly light colored quality in the samples originating from RNAi lines. Together with similar effects seen in previously published experiments involving other potato varieties, the Desiree results described here establish the efficacy of using RNAi for the successful reduction of CIS in potato tubers.

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