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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.9
- 수록면
- 669 - 679 (11page)
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N-acetyl-D-glucosamine kinase (GlcNAc kinase or NAGK) primarily catalyzes phosphoryl transfer to GlcNAc during amino sugar metabolism. Recently, it was shown NAGK interacts with dynein light chain roadblock type 1 (DYNLRB1) and upregulates axo-dendritic growth, which is an enzyme activity-independent, non-canonical struc-tural role. The authors examined the distributions of NAGK and NAGK-dynein complexes during the cell cycle in HEK293T cells. NAGK was expressed throughout different stages of cell division and immunocytochemistry (ICC) showed NAGK was localized at nuclear envelope, spindle microtubules (MTs), and kinetochores (KTs). A proximity ligation assay (PLA) for NAGK and DYNLRB1 revealed NAGK-dynein complex on nuclear envelopes in prophase cells and on chromosomes in metaphase cells. NAGK-DYNLRB1 PLA followed by Lis1/NudE1 immunostaining showed NAGK-dynein complexes were colocalized with Lis1 and NudE1 signals, and PLA for NAGK-Lis1 showed similar signal patterns, suggesting a functional link between NAGK and dynein-Lis1 complex. Subsequently, NAGK-dynein complexes were found in KTs and on nuclear membranes where KTs were marked with CENP-B ICC and nuclear membrane with lamin ICC. Furthermore, knockdown of NAGK by small hairpin (sh) RNA was found to delay cell division. These results indicate that the NAGK-dynein interaction with the involvements of Lis1 and NudE1 plays an important role in prophase nuclear envelope breakdown (NEB) and metaphase MT-KT attachment during eukaryotic cell division.
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참고문헌 (58)
참고문헌 신청
Beaudouin, J. / 2002 / Nuclear envelope breakdown proceeds by microtubuleinduced tearing of the lamina / Cell 108:83~96
Bolhy, S. / 2011 / A Nup133-dependent NPC-anchored network tethers centrosomes to the nuclear envelope in prophase / J. Cell Biol 192:855~871
Cockell, M. M. / 2004 / Lis-1 is required for dynein-dependent cell division processes in C. elegans embryos / J. Cell Sci 117:4571~4582
Coquelle, F. M. / 2002 / LIS1, CLIP-170's key to the dynein/dynactin pathway / Mol. Cell. Biol 22:3089~3102
Datta, A. / 1970 / Studies on hog spleen N-acetylglucosamine kinase. I. Purification and properties of N-acetylglucosamine kinase / Biochim. Biophys. Acta 220:51~60
Bolhy, S. / 2011 / A Nup133-dependent NPC-anchored network tethers centrosomes to the nuclear envelope in prophase / J. Cell Biol 192:855~871
Cockell, M. M. / 2004 / Lis-1 is required for dynein-dependent cell division processes in C. elegans embryos / J. Cell Sci 117:4571~4582
Coquelle, F. M. / 2002 / LIS1, CLIP-170's key to the dynein/dynactin pathway / Mol. Cell. Biol 22:3089~3102
Datta, A. / 1970 / Studies on hog spleen N-acetylglucosamine kinase. I. Purification and properties of N-acetylglucosamine kinase / Biochim. Biophys. Acta 220:51~60
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