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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제12호
- 발행연도
- 2016.12
- 수록면
- 2,051 - 2,059 (9page)
- DOI
- 10.4014/jmb.1609.09062
이용수
초록· 키워드
오류제보하기
To date, there has been no employment of a magnetoelastic (ME) biosensor method to detect Salmonella enterica serovar Typhimurium in soil. The ME biosensor method needs to be investigated and modified for its successful performance. The filtration method, cationexchange resin method, and combinations of both methods were employed for the extraction of S. Typhimurium from soil. The number of S. Typhimurium and the resonant frequency shift of the ME sensor were then compared using a brilliant green sulfa agar plate and an HP 8751A network analyzer. A blocking study was performed using bovine serum albumin (BSA), polyethylene glycol (PEG), and casein powder suspension. Finally, the modified ME biosensor method was performed to detect S. Typhimurium in soil. The number of S. Typhimurium was significantly decreased from 7.10 log CFU/soil to 4.45-4.72 log CFU/soil after introduction of the cation-exchange resin method. The greatest resonant frequency shift of the measurement sensor was found when employing centrifugation and filtration procedures. The resonant frequency shift of the PEG-blocked measurement sensor was 3,219 ± 755 Hz, which was significantly greater than those of the BSA- and casein-blocked ME sensor. The optimum concentration of PEG was determined to be 1.0 mg/ml after considering the resonant shift and economic issue. Finally, the modified ME biosensor method was able to detect S. Typhimurium in soil in a dose-response manner. Although these modifications of the ME biosensor method sacrificed some advantages, such as cost, time effectiveness, and operator friendliness, this study demonstrated a novel approach of the ME biosensor method to detect S. Typhimurium in soil.
목차
등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
Anderson M / 2011 / Pathogen-produce pair attribution risk ranking tool to prioritize fresh produce commodity and pathogen combinations for further evaluation(P3ARRT) / Food Control 22:1865~1872
Berger CN / 2010 / Fresh fruit and vegetables as vehicles for the transmission of human pathogen / Environ. Microbiol 12:2385~2397
Beuchat LR / 2002 / Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables / Microbes Infect 4:413~423
Beuchat LR / 2006 / Vectors and conditions for preharvest contamination of fruits and vegetagles with pathogens capable of causing enteric diseases / Br. Food J 108:38~53
Byeon HM / 2015 / Lytic phage-based magnetoelastic biosensors for on-site detection of methicillin-resistant Staphylococcus aureus on spinach leaves / J. Electrochem. Soc 162:B230~B235
Berger CN / 2010 / Fresh fruit and vegetables as vehicles for the transmission of human pathogen / Environ. Microbiol 12:2385~2397
Beuchat LR / 2002 / Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables / Microbes Infect 4:413~423
Beuchat LR / 2006 / Vectors and conditions for preharvest contamination of fruits and vegetagles with pathogens capable of causing enteric diseases / Br. Food J 108:38~53
Byeon HM / 2015 / Lytic phage-based magnetoelastic biosensors for on-site detection of methicillin-resistant Staphylococcus aureus on spinach leaves / J. Electrochem. Soc 162:B230~B235
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