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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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CD44 is a particular adhesion molecule and facilitates both cell-cell and cell-matrixinteractions. In particular, splice variants of CD44 are particularly overexpressed in a large numberof malignancies and carcinomas. In this study, the 177Lu-labelled CD44 targeting antibodywas prepared and bioevaluated in vitro and in vivo. Anti-CD44 was immunoconjugated with theequivalent molar ratio of cysteine-based DTPA-NCS and radioimmunoconjugated with 177Lu atroom temperature within 15 minutes. The stability was tested in human serum. An in vitro studywas carried out in HT-29 human colon cancer cell lines. For the biodistribution study 177Lu-labelledanti-CD44 was injected in xenograft mice. Anti-CD44 was immunoconjugated with cysteinebasedDTPA-NCS and purified by a centricon filter system having a molecular cut-off of 50kDa. Radioimmunoconjugation with 177Lu was reacted for 15 min at room temperature. Theradiolabeling yield was >99%, and it was stable in human serum without any fragmentation ordegradation. The radioimmunoconjugate showed a high binding affinity on HT-29 colon cancer cellsurfaces. In a biodistribution study, the tumor-to-blood ratio of the radioimmunoconjugate was43 : 1 at 1 day post injection (p.i) in human colon cancer bearing mice. The anti-CD44 monoclonalantibody for the targeting of colon cancer was effectively radioimmunoconjugated with 177Lu.
The in vitro high immunoactivity of this radioimmunoconjugate was determined by a cell bindingassay. In addition, the antibody’s tumor targeting ability was demonstrated with very high uptakein tumors. This radioimmunoconjugate is applicable to therapy in human colon cancer withhighly expressed CD44.
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참고문헌 (22)
참고문헌 신청
Boswell CA / 2007 / Development of radioimmunotherapeutic and diagnostic antibodies : an inside-out view / Nucl. Med. Biol 34:757~778
Kang-hyuk Choi / 2006 / Preparation of an Amino Acid Based DTPA as a BFCA for Radioimmunotherapy / Bulletin of the Korean Chemical Society 27(8):1194~1198
최선주 / 2006 / 치료용 방사성동위원소 / Nuclear Medicine and Molecular Imaging 40(2):58~65
Gadhoum Z / 2004 / The effect of anti-CD44 monoclonal antibodies on differentiation and proliferation of human acute myeloid leukemia cells / Leuk Lymphoma 45:1501~1510
Goldenberg DM / 2006 / Advances in cancer therapy with radiolabeled monoclonal antibodies / Q. J. Nucl. Med. Mol. Imaging 50:248~264
Kang-hyuk Choi / 2006 / Preparation of an Amino Acid Based DTPA as a BFCA for Radioimmunotherapy / Bulletin of the Korean Chemical Society 27(8):1194~1198
최선주 / 2006 / 치료용 방사성동위원소 / Nuclear Medicine and Molecular Imaging 40(2):58~65
Gadhoum Z / 2004 / The effect of anti-CD44 monoclonal antibodies on differentiation and proliferation of human acute myeloid leukemia cells / Leuk Lymphoma 45:1501~1510
Goldenberg DM / 2006 / Advances in cancer therapy with radiolabeled monoclonal antibodies / Q. J. Nucl. Med. Mol. Imaging 50:248~264
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