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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2023.6
- 수록면
- 43 - 50 (8page)
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초록· 키워드
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Canine parvovirus (CPV) is highly contagious and causes acute hemorrhagic gastroenteritis in puppies. Four variants of CPV-2 are widely distributed worldwide: CPV-2, CPV-2a, CPV-2b, and CPV-2c. However, there have been few molecular characterization studies of CPV-2a circulating in dog populations in South Korea. Here, we isolated and characterized two CPVs: the CPV2101 and CPV2102 isolates. They were confirmed to be CPVs based on their cytopathic effects, indirect fluorescence assays, electron microscopy, real-time PCR, and sequence analysis. Both isolates showed hemagglutination activity with pig erythrocytes and had viral titers ranging from 10<SUP>6.5</SUP> to 10<SUP>5.5</SUP> FAID50/mL in A72 cells. The nucleotide and amino acid sequences of VP2 of the isolates were compared with reported CPV strains. The sequence alignment showed that they had 100% nucleotide sequence similarity with each other and grouped with CPV-2a. They had mutations at positions 267, 322, 324, and 440 in the VP2 gene compared to the original CPV-2a strains. A phylogenetic analysis of the VP2 gene of the isolates revealed that they were closely related to the K01708-01 strain reported from South Korea. The two isolates will serve as useful antigens for developing new CPV vaccines.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
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UCI(KEPA) : I410-ECN-0102-2023-475-001917881