지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.6
- 수록면
- 119 - 126 (8page)
- DOI
- 10.17519/apiculture.2024.06.39.2.119
이용수
초록· 키워드
오류제보하기
Isothermal amplification methods have become a cornerstone for pathogen detection, offering a simplified alternative to the polymerase chain reaction by enabling target gene amplification at a steady temperature. This study focuses on recombinase polymerase amplification (RPA), an isothermal technique capable of amplifying a target gene within 20 to 30 minutes at a constant 37°C. Leveraging this approach, we sought to devise a rapid and precise diagnostic method for the deformed wing virus (DWV) in honey bees. By incorporating reverse transcriptase into the TwistAmp<SUP>®</SUP> Basic kit, we facilitated one-step RT-RPA for DWV detection. Prior to this, we meticulously designed a suitable primer set and fine-tuned the reaction conditions, varying temperature, duration, primer selection, and magnesium ion concentration to optimize the process. Our newly developed diagnostic method streamlines nucleic acid separation by utilizing the Quick Extract<SUP>TM</SUP> DNA Extraction Solution. As a result, the entire process-from extraction to diagnosis-now takes just 70 minutes. Moreover, this method’s simplicity enables on-site field diagnosis, enhancing its practical application for immediate DWV detection in honey bees.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
LITERATURE CITED
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Evaluation and Application of DNA Chip using recombinase polymerase amplification for detection against honey bee pathogens
한국양봉학회 학술대회 자료집
2016 .10
-
꿀벌 병원성 바이러스 Deformed Wing Virus의 4종 특이 단백질들의 대량 생산
Journal of Apiculture
2015 .11
-
Rapid and sensitive detection of Plum pox virus using isothermal reverse transcription-recombinase polymerase amplification
한국농약과학회 학술발표대회 논문집
2018 .10
-
Rapid and specific detection of Apple stem grooving virus by reverse transcription-recombinase polymerase amplification
한국농약과학회 학술발표대회 논문집
2018 .10
-
Simultaneous detection of cucumber viruses by reverse transcription recombinase polymerase amplification assay
한국농약과학회 학술발표대회 논문집
2018 .10
-
Molecular Detection of Honey Bee Pathogenic Microbes: Recent Advances and Future Perspective
Journal of Apiculture
2021 .06
-
Rapid, sensitive, and specific detection of Apple stem pitting virus in pear plants by reverse transcription-recombinase polymerase amplification assay
한국농약과학회 학술발표대회 논문집
2018 .10
-
Honey Bee Viruses: An Ongoing History of Discovery
Journal of Apiculture
2023 .06
-
역전사 실시간 Recombinase Polymerase Amplification (RT/RT RPA)에 의한 꿀벌 Black Queen Cell Virus의 신속 검출
Journal of Apiculture
2016 .04
-
Rapid Detection of Black Queen Cell Virus from Honeybee by Real-Time Recombinase Polymerase Amplification
한국양봉학회 학술대회 자료집
2016 .03
-
재조합효소-중합효소 증폭법(RPA) 및 중합효소 연쇄반응법(PCR)에서 생성된 특이 DNA 증폭산물 간의 비교
Journal of Apiculture
2017 .04
-
일천궁에 발생하는 오이모자이크바이러스의 현장 검출을 위한 Lateral Flow Reverse Transcription Recombinase Polymerase Amplification (LF-RT-RPA) 개발
한국약용작물학회지
2023 .12
-
Development of Detection-method against Korean Sacbrood Virus (kSBV) using Real-time sothermal Recombinase Polymerase Amplification (RPA)
한국양봉학회 학술대회 자료집
2016 .03
-
환경친화적 식량생산을 위한 꿀벌의 다원적 가치
식품과학과 산업
2022 .06
-
Nutrition for Honey Bee to Prevent Colony Collapse
Journal of Apiculture
2022 .11
-
Rapid detection of Peronospora destructor using Recombinase polymerase amplification (RPA) combined with bridging flocculation assay
한국농약과학회 학술발표대회 논문집
2018 .10
-
Antiviral Effect of Korean Native Bee-honey against Influenza A Virus in A549 Cells
Journal of Apiculture
2023 .11
-
꿀벌 병원성 바이러스의 정량적 검출을 위한 정량 실시간 재조합효소-중합효소 증폭법의 개발
Journal of Apiculture
2016 .06
-
Development of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of Plum pox virus isolated from peach in Korea
한국농약과학회 학술발표대회 논문집
2018 .10
-
Expectations about the Potential Impacts of Climate Change on Honey Bee Colonies in Egypt
Journal of Apiculture
2016 .06
이 논문과 함께 이용한 논문
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-151-24-02-090048672