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논문 기본 정보

자료유형
학술저널
저자정보
Wang Tong (Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University) Song Yajuan (Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University) Yang Liu (Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University) Liu Wei (Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University) He Zhen’an (Shaanxi Institute of Medical Device Quality Inspection) Shi Yi (Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University) Song Baoqiang (Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University) Yu Zhou (Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University)
저널정보
한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제21권 제1호
발행연도
2024.1
수록면
65 - 79 (15page)
DOI
10.1007/s13770-023-00601-5

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초록· 키워드

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Background: Cutaneous wound healing represents a common fundamental phenomenon requiring the participation of cells of distinct types and a major concern for the public. Evidence has confirmed that photobiomodulation (PBM) using near-infrared (NIR) can promote wound healing, but the cells involved and the precise molecular mechanisms remain elusive. Methods: Full-thickness skin defects with a diameter of 1.0 cm were made on the back of rats and randomly divided into the control group, 10 J, 15 J, and 30 J groups. The wound healing rate at days 4, 8, and 12 postoperatively was measured. HE and Masson staining was conducted to reveal the histological characteristics. Immunofluorescence staining was performed to label the epidermal stem cells (ESCs) and hair follicle stem cells (HFSCs). Western blot was performed to detect the expressions of proteins associated with ESCs and HFSCs. Cutaneous wound tissues were collected for RNA sequencing. Gene ontology and the Kyoto Encyclopedia of Genes and Genomes analysis was performed, and the hub genes were identified using CytoHubba and validated by qRT-PCR. Results: PBM can promote reepithelialization, extracellular matrix deposition, and wound healing, increase the number of KRT14+/PCNA+ ESCs and KRT15+/PCNA+ HFSCs, and upregulate the protein expression of P63, Krt14, and PCNA. Three hundred and sixty-six differentially expressed genes (DEGs) and 7 hub genes including Sox9, Krt5, Epcam, Cdh1, Cdh3, Dsp, and Pkp3 were identified. These DEGs are enriched in skin development, cell junction, and cadherin binding involved in cell–cell adhesion etc., while these hub genes are related to skin derived stem cells and cell adhesion. Conclusion: PBM accelerates wound healing by enhancing reepithelialization through promoting ESCs and HFSCs proliferation and elevating the expression of genes associated with stem cells and cell adhesion. This may provide a valuable alternative strategy to promote wound healing and reepithelialization by modulating the proliferation of skin derived stem cells and regulating genes related to cell adhesion.

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