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논문 기본 정보

자료유형
학술저널
저자정보
김효연 (단국대학교 의과대학 이비인후-두경부외과학교실) 모지훈 (단국대학교 의과대학 이비인후-두경부외과학교실) 유신혁 (단국대학교 의과대학 이비인후-두경부외과학교실) 변광현 (단국대학교 의과대학 이비인후-두경부외과학교실)
저널정보
대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후-두경부외과학회지 제67권 제2호
발행연도
2024.2
수록면
79 - 86 (8page)
DOI
https://doi.org/10.3342/kjorl-hns.2023.00332

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Background and Objectives There are various methods used to histologically assess thecell count of neutrophils in nasal polyps. With various classification of neutrophilic nasal pol-yps, determining the appropriate staining techniques and criteria is deemed important for thediagnosis of neutrophilic nasal polyps by assessing neutrophil cell counts. In this study, wetried to optimize different methods of neutrophil counting and staining by comparing clinicaloutcomes. Subjects and Method We studied 207 patients who underwent endoscopic sinus surgeryfor chronic rhinosinusitis with nasal polyp from August 2014 to September 2018. Patients’ na-sal polyp were taken to make following three slide sections: hematoxylin and eosin, humanneutrophil elastase (HNE), myeloperoxidase. After staining, microscope picture (×400) wastaken and neutrophils were counted. Neutrophil polyp (NP) was classified either by the tissueneutrophil count (20/HPF) or by the neutrophil percentage (1%/total cell) for clinical outcomecomparison. Results Two groups were made after HNE staining: the NP and the non-NP groups. TheL-K scores and delta showed significant differences between each group while neutrophil wasdefined based on 20 counts/HPF ( p<0.001). By Kaplan-Meier estimation, there was no statis-tical meaning between the two groups. But the NP group had a tendency to recur more thanthe non-NP group while 20 counts/HPF definition was used ( p>0.05). Conclusion HNE staining and 20 counts/HPF were the most specific for neutrophil count-ing. Analyzing the nasal polyp into two groups due to histologic types showed that the NPgroup had worse prognosis than the non-NP group. In conclusion, neutrophils in the nasal pol-yp might be considered seriously for clinical outcomes.

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