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논문 기본 정보

자료유형
학술저널
저자정보
박수진 (남서울대학교) 배현숙 (남서울대학교)
저널정보
대한예방치과·구강보건학회 대한구강보건학회지 대한구강보건학회지 제48권 제2호
발행연도
2024.6
수록면
33 - 39 (7page)
DOI
https://doi.org/10.11149/jkaoh.2024.48.2.33

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Objectives: In this study, we investigated the validity and effectiveness of biomarkers and oral microbiome analysis for evaluation of periodontal status following scaling intervention. Methods: The study included 15 participants who were selected based on inclusion/exclusion criteria. Periodontal indices, including the plaque index, probing depth, modified sulcus bleeding index,and calculus index were measured to evaluate clinical periodontal status and disease progression. Pre- and post-scaling gingival crevicular fluid (GCF) was obtained from 12-20 sites per patient tomeasure changes in the odontogenic ameloblast-associated protein (ODAM) concentration using anenzyme-linked immunosorbent assay. Additionally, pre- and post-treatment salivary samples wereobtained for quantitative analysis of oral microbiome distribution. Results: All clinical parameters showed significant differences in pre- and post-scaling valuesand significant changes in the ODAM concentration in the GCF in patients with initial and moderate periodontitis. Quantitative analysis performed after scaling intervention revealed changes ineight oral disease-causing bacteria associated with periodontal disease, including Aggregatibacter,Porphyromonas, Tannerella, Treponema, Prevotella, and Fusobacterium. Specifically, Tannerellaforsythia and Fusobacterium nucleatum (well-known major periodontal pathogens) numbers weresignificantly decreased following non-surgical periodontal therapy. However, no significant association was observed between changes in the salivary oral microbiota and ODAM concentration in theGCF, following treatment. Conclusions: Based on the findings of this study, we propose the use of ODAM protein and quantitative analysis of periodontal disease-associated bacteria as objective and quantitative biomarkersfor evaluation of improvement in periodontal disease following scaling intervention.

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